微生物对抗剂作为生物杀虫剂,用于控制由Xanthomonas oryzae pv.引起的细菌叶病. 米种是米种的一种
Adyasha Anapurba Sahoo1, Preeti Pallavi1, Pragnya Paramita Sahoo1
1School of Pharmaceutical Sciences, Centre for Biotechnology, Siksha 'O' Anusandhan (Deemed to be University), Bhubaneswar, Odisha, India.
Journal of basic microbiology
|October 22, 2025
概括
研究人员确定了三种细菌分离物,Alcaligenes faecalis AJS36,Enterobacter hormaechei ACS17和Enterobacter asburiae ACS48,作为有效的生物控制剂,用于对抗大米中的细菌叶病 (BLB). 这些药物显著降低了疾病的严重程度,并为化学农药提供了可持续的替代品.
科学领域:
- 微生物学 微生物学
- 植物病理学 植物病理学
- 生物技术是生物技术.
背景情况:
- 细菌叶病 (BLB),是由Xanthomonas oryzae pv.引起的 (Xoo) 是对全球大米生产的重大威胁.
- 目前的管理策略通常依赖于化学农药,需要开发可持续的替代品.
研究的目的:
- 针对Xoo. 的新型细菌生物控制剂的分离和表征.
- 评估已识别的分离物在抑制Xoo生长和毒性因子方面的有效性.
- 评估这些药物在Oryza sativa L.中的BLB管理中的实地应用潜力.
主要方法:
- 隔离和选115个细菌分离物,以检测它们对Xoo.的对抗活性.
- 使用16S rRNA基因测序识别有力的分离物.
- 确定最小抑制度 (MIC) 并评估对生物膜形成,桑托蒙纳丁产量,EPS合成,细胞蛋白泄漏和清理活动的影响.
- 对抗生素敏感性进行分析.
- 温室试验评估了叶子应用对BLB的疗效.
主要成果:
- 三个分离物,被确定为Alcaligenes faecalis AJS36,Enterobacter hormaechei ACS17和Enterobacter asburiae ACS48,对Xoo.表现出显著的对抗活性.
- 隔离剂AJS36和ACS17有效地降低了Xoo的生物膜形成,桑托莫纳丁生产和EPS合成,同时还诱导了蛋白质泄漏和增强了清理活动.
- 在温室试验中,这些分离物的叶子应用,特别是AJS36,显著减少了病变长度和疾病严重程度.
结论:
- A. faecalis AJS36,E. hormaechei ACS17和E. asburiae ACS48是水中BLB可持续管理的有希望的生物控制候选者.
- 这些隔离物提供了一个环保的替代品,用于控制化学农药Xoo.
- 这项研究代表了关于使用这些特定隔离物在米植物中用于BLB管理的第一份报告.
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