两个保存序列基因之间的协作驱动由Aha1对Hsp90的ATPase刺激
Desmond Prah Amoah1, Rebecca Mercier1, Gnin Alyousef1
1Department of Cell Biology, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, Alberta, Canada.
Protein science : a publication of the Protein Society
|October 22, 2025
概括
Aha1的共同主持人之一.
科学领域:
- 分子生物学分子生物学
- 蛋白质折叠过程中的蛋白质折叠
- 生物化学 生物化学
背景情况:
- 热冲击蛋白90 (Hsp90) 是一个对细胞功能至关重要的分子伴侣.
- 像Aha1这样的协伴蛋白调节Hsp90的ATPase循环和客户端蛋白质的成熟.
- Aha1含有保留的NxNNWHW和RKxK动机,影响Hsp90的活动.
研究的目的:
- 研究K60残留在Aha1的RKxK基因中的作用.
- 确定Aha1的NxNNWHW基因残留如何影响Hsp90 ATPase活性.
- 阐明Hsp90功能中的Aha型辅助子的调节机制.
主要方法:
- 酵母的局部导向突变发生 Aha1.1.
- 突变酵母的体内功能测试 Aha1.1.
- 对Hsp90 ATPase动态的生物化学分析.
主要成果:
- 在RKxK基因中K60的突变在体内部分损害了酵母Aha1的功能.
- 在ATP水解之前,K60残留物促进了NxNNWHW基因的结构组织.
- 在NxNNWHW基因中的每个残留物差异地调节Hsp90的ATPase速率和ATP亲和力.
结论:
- K60残留物对Aha1的辅助子功能和Hsp90的调节很重要.
- 在Aha1的保存基因中的特定残留微调Hsp90的ATPase活性.
- 这些发现提高了对Hsp90介导的客户端蛋白折叠调节的理解.
相关概念视频
Energy to Drive Translocation
2.7K
Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...
Generally, polypeptides are unfolded by two distinct...
2.7K
ATP Synthase: Mechanism
16.7K
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
16.7K
Allosteric Proteins-ATCase
6.5K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
6.5K
ATP Synthase: Structure
15.1K
ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
15.1K
Translocation of Proteins into the Mitochondria
12.1K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
12.1K
ATP Driven Pumps III: V-type Pumps
4.6K
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
4.6K


