加强对致病性维布里奥菌种的控制. 在水产养殖中使用能够破坏宿主范围以外的生物膜的菌体
Ni Wang1,2, Chengcheng Li2,3, Jiulong Zhao2,3
1College of Marine Life Sciences, MoE Laboratory of Evolution and Marine Biodiversity, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, China.
Applied and environmental microbiology
|October 22, 2025
概括
两种新型细菌菌体VpT和VpR有效地对抗水产养殖中的多抗药性Vibrio parahaemolyticus生物膜. 这些菌体降解了多样化的Vibrio生物膜,并提高了抗微生物药物的有效性,为水产养殖病原体控制提供了有希望的解决方案.
科学领域:
- 水产养殖病理学 水产养殖病理学
- 微生物生态学 微生物生态学
- 病毒学 病毒学
背景情况:
- 菌种对水产养殖业构成重大威胁,由于它们具有弹性,多种生物膜,造成了相当大的经济损失.
- 传统的抗微生物药物往往对这些生物膜无效,需要使用替代的控制策略.
- 细菌菌体是一个有希望的替代品,但强大的抗菌膜菌体对抗Vibrio仍然很少.
研究的目的:
- 隔离和描述具有抗生素膜活性的新型液性细菌对抗多抗药性Vibrio parahaemolyticus的新型液性细菌.
- 评估菌体在预防,破坏和降解预制Vibrio生物膜的有效性,包括复杂的多种群体.
- 研究菌体的协同作用潜力,当作为尾酒用于增强抗微生物疗效和抑制细菌再生.
主要方法:
- 隔离和表征两个新型的菌体,VpT和VpR,针对多药性耐药性Vibrio parahaemolyticus.
- 基因组和遗传学分析以分类分离的菌体.
- 在体外测试以评估生物膜的预防,破坏和降解能力,针对各种Vibrio物种和混合物种生物膜.
- 评估菌体尾酒与抗微生物药物结合的疗效,以评估协同效应和抑制细菌再生.
主要成果:
- 两种新型的菌体VpT和VpR被分离和特征化,属于一种新型病毒家族和Caudoviricetes类中的物种.
- 这两种菌体都表现出强大的生物膜预防和破坏对宿主菌株的作用,并有效降解了八种非宿主Vibrio物种和复杂的多物种Vibrio群体的预制生物膜.
- 一种由VpT和VpR组成的菌体尾酒通过抑制细菌再生来表现出协同的抗菌疗效,解决了菌体治疗的关键局限性.
结论:
- 在水产养殖中,VpT和VpR菌体是有效的生物控制剂,可以对抗Vibrio感染和与生物膜相关的挑战.
- 它们的宿主范围独立的跨物种生物膜降解活动使它们适合控制复杂的多微生物生物膜.
- VpT和VpR的协同作用增强了抗微生物药物的有效性,为打击水产养殖病原体提供了一种新的策略.
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