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Updated: Jan 14, 2026

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Published on: June 10, 2013

LncRNA MYL2 作为 miR-661 的海绵,调节术后认知功能障碍

Yiguo Xu¹, Xiaoyun He², Huijun Zhang²

¹Anesthesiology Department, The First Affiliated Hospital of Ningbo University, No. 59, Liuting Street, Ningbo, 315000, Zhejiang Province, China. xuyiguo66688@163.com.

Journal of molecular neuroscience : MN

|October 22, 2025

概括

在老老鼠中,术后认知功能障碍 (POCD) 由于 lncRNA MYL2 的增加而恶化,从而增加神经炎症和氧化应激. 恢复miR-661水平可以减轻这些有害影响,提供潜在的治疗点.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
老年学是一门学科.

背景情况:

手术后认知功能障碍 (POCD) 显著影响老年患者的生活质量,其分子机制尚不清楚.
全球近50%的老年人口面临POCD风险.

研究的目的:

在POCD.老鼠模型中研究长非编码RNA (lncRNA) MYL2的表达.
阐明MYL2对POCD认知功能,神经炎症和氧化应激的影响.
探索MYL2和microRNA (miRNA) miR-661作为潜在的治疗点之间的关系.

主要方法:

在老老鼠中建立了一个POCD模型,使用骨切除术和sevoflurane麻醉.
使用莫里斯水迷宫 (MWM) 评估认知功能.
通过ELISA测量了炎症性细胞因子 (TNF-α,IL-6,IL-1β) 和氧化应激标志物.
使用了光酶记者测定来确认MYL2和miR-661.1之间的相互作用.
使用CCK-8和流细胞计评估了细胞活力和细胞亡.

主要成果:

治疗sevoflurane上调了老鼠海马中的MYL2表达.
过度表达MYL2导致认知功能受损,通过增加逃逸延迟和减少目标象限入口来证明这一点.
随着MYL2过度表达,观察到高水平的促炎细胞因子和氧化应激标志物.
MYL2作为miR-661的海绵,miR-661的干预可以逆转MYL2对POCD的有害影响.

结论:

在POCD中,高调节的IncRNA MYL2会加剧神经炎症,氧化损伤和细胞功能障碍.
MYL2与miR-661的相互作用为管理POCD提供了一个新的治疗途径.
针对MYL2/miR-661轴可能为治疗老年人POCD提供一个有希望的策略.

关键词:

认知功能障碍认知功能障碍在LncRNA MYL2中.神经炎症是一种神经炎症.西沃氨酸是什么?在 miR-6611,它是最好的.

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...