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商业合银中的银离子毒性:来自Huh-7细胞高含量成像的分子见解

Bruno L Menna¹, Mila Simić¹, Ljubica Tasic²

¹Departamento de Bioquímica e Biologia Tecidual, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, Brasil.

|October 22, 2025

概括

商用合银补充剂对人类肝细胞具有显著的毒性. 该研究发现,液体中的银离子,而不是纳米颗粒,是细胞损伤的主要原因,这引发了安全问题.

科学领域:

生物化学生物化学
毒理学毒理学毒理学
纳米技术纳米技术

背景情况:

体银作为食补充剂被广泛推销.
它所谓的健康益处缺乏科学验证,潜在的毒性令人担忧.
需要了解商业合银产品对细胞的影响.

研究的目的:

评估两种商业合银配方 (Almacura和Prata Vida) 对人类肝瘤细胞系 Huh-7 的细胞毒性作用.
确定银离子 (Ag+) 和银纳米粒子 (AgNPs) 对观察到的细胞毒性的贡献.
为了比较不同细胞活力测定和高含量成像测试的灵敏度,以评估合银的毒性.

主要方法:

用MTT测定,细胞计数 (PI/Hoechst染色) 和膜完整性测定 (PI吸收) 来评估细胞毒性.
这项研究区分了上位物 (Ag+离子) 和颗粒物 (AgNPs) 对细胞毒性的影响.
使用活细胞绘画试验的高含量成像用于剂量反应评估.

主要成果:

无论是Almacura还是Prata Vida配方都表现出显著的细胞毒性,在不同的测定中,IC50值通常低于1μg/mL.
主要含有银离子 (Ag+) 的超被确定为细胞毒性的主要驱动因素.
高含量成像显示细胞变化在低度比传统的生存能力测试.

结论:

商业合银配方由于对人类肝细胞的细胞毒性而带来重大安全风险.
银离子,而不是纳米粒子,是观察到的毒性的主要贡献者.
这些发现强调了对合银产品的严格监管标准的必要性,考虑到纳米粒子特性和生物相互作用.

关键词:

在 Ag+ Ag+ 的情况下.合性银纳米粒子的结合性银纳米粒子.高内容成像成像技术纳米毒性纳米毒性

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