经过基因工程改造的植物内细胞扩大了由效应器触发的免疫力
Menglu Hou1, Sitao Zhu1, Ruixia Niu1
1State Key Laboratory of Hybrid Rice, Hubei Provincial Research Center for Basic Biological Sciences, Hubei Hongshan Laboratory, RNA Institute, Institute for Advanced Studies (IAS), Wuhan University, Wuhan, Hubei 430072, China.
Cell host & microbe
|October 22, 2025
概括
被称为"哨兵"的工程细菌通过表达病原体因子来激活植物免疫力. 这种新的策略增强了植物对各种病原体的防御能力,补充了现有的微生物方法.
科学领域:
- 植物病理学 植物病理学
- 微生物学 微生物学
- 合成生物学 合成生物学
背景情况:
- 植物使用核酸结合的氨酸丰富重复 (NLR) 受体进行对病原体的效应器触发免疫 (ETI).
- ETI的有效性受到病原体效应者识别的限制,这在自然感染中是不可预测的.
研究的目的:
- 设计植物内细胞 (哨兵) 来表达病原体效应体并激活ETI.
- 开发一种广泛的植物疾病耐药性的多功能战略.
主要方法:
- 工程内植物 (哨兵) 以异质表达由宿主NLRs识别的病原因因子.
- 利用OxyR调节电路来触发由活性氧物种 (ROS) 触发的效应体表达.
- 在不同的植物中对多种病原体测试了各种效应器-NLR对.
主要成果:
- 哨兵殖民活动激活了对缺乏可识别的效应因子的病原体的ETI.
- 由ROS触发的表达使ETI激活成为可能.
- 尽管有哨兵殖民,但微生物群的多样性和植物生长仍然保持不变.
结论:
- 工程哨兵为宽频ETI激活提供了一个多功能平台.
- 这一策略利用微生物群-宿主-病原体相互作用来增强植物防御.
- 补充了合成微生物联盟,以进行强大的植物疾病管理.
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