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在Drosophila精子生成中,Ssrp控制了独立于FACT复合体的生殖线发育
Xiaoning Tan1,2, Yuhan Lou1, Zhiyong Yin3,4,5
1Division of Human Reproduction and Developmental Genetics, The Women's Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, Zhejiang, China.
Cell & bioscience
|October 23, 2025
概括
FACT复合体子单元Ssrp在果的丸中具有双重作用,支持体细胞并独立调节生殖细胞分裂和半分裂. 由于破坏细胞周期和染色体稳定性,Ssrp的损失导致男性不育.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 染色体生物学 染色体生物学
背景情况:
- 这种FACT复合体 (SSRP1/Ssrp和SUPT16H/Dre4) 被称为染色体重塑剂.
- 事实上,复杂突变与智力障碍有关,它维持神经干细胞命运.
- 人体丸生殖细胞中FACT复合体表达高,但其在精子生成中的作用尚不清楚.
研究的目的:
- 使用Drosophila melanogaster,研究精子生成过程中FACT复合物的功能.
- 确定Ssrp和Dre4在丸体细胞和生殖细胞中的特定作用.
- 阐明在男性生殖细胞发育中的FACT复合体参与的分子机制.
主要方法:
- 在Drosophila中进行了Ssrp和dre4的生殖系特异性和体内特异性淘汰.
- 利用免疫覆盖来评估细胞功能和缺陷.
- 在缺乏Ssrp的丸上进行了批量和单细胞RNA测序,以分析转录组变化.
主要成果:
- Ssrp的功能取决于细胞环境:它与体细胞中的Dre4合作,通过糖解维持干细胞.
- 生殖线Ssrp独立于Dre4运作,控制过渡放大分裂和半分裂.
- 生殖系Ssrp的枯竭导致男性不育,精子积累,线粒错误和介质停止,与中粒体凝聚力受损和能量代谢改变有关.
结论:
- 在Drosophila精子生成过程中,Ssrp在体内和生殖线两部分都起着重要且取决于环境的作用.
- Ssrp是生殖细胞增殖,介质性进展和染色体稳定性的关键调节者.
- 这些发现提供了与Ssrp功能障碍相关的生殖障碍的见解,并突出了FACT复合体在男性生殖细胞发育中的重要性.
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