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在两种跨大陆皮卡物种中,βglu-1的分子表型变化,βglu-1是一种与昆虫防御相关的β-葡萄糖酶基因

Ernest T Y Wu¹, Tin Hang Hung¹, Nadir Erbilgin²

¹Department of Biology, University of Oxford, Oxford, UK.

Molecular ecology

|October 23, 2025

概括

杉树中β-葡萄糖酶-1 (βglu-1) 的基因表达影响了对昆虫的防御化合物. βglu-1转录水平的变化与环境因素和Picea物种中的昆虫爆发有关.

科学领域:

植物生物学植物生物学
森林生态森林生态学
化学生态化学生态学

背景情况:

森林树木依赖于对抗昆虫害虫的防御机制.
在Picea glauca中β-glucosidase-1 (βglu-1) 基因表达产生乙烯,防御Choristoneura fumiferana.
在Picea abies中也存在βglu-1,尽管其本土范围中没有主要的昆虫除叶者.

研究的目的:

为了比较Picea glauca (北美) 和Picea abies (欧洲) 之间的βglu-1转录水平的范围广泛变化.
调查βglu-1转录水平与环境因素之间的相关性,包括昆虫害虫的存在和气候变量.

主要方法:

使用定量实时PCR (RT-qPCR) 来测量βglu-1转录水平.
向转录组测序提供了基因表达的补充数据.
在两种Picea物种的原生范围收集了叶子样本.

主要成果:

对Picea两种物种来说,βglu-1转录水平在种群内和种群之间显示出显著的变化.
一种βglu-1基因形式在Picea glauca中显著差异地表达.
在Picea glauca中,βglu-1转录水平与纵向变化,年平均降水量和Choristoneura fumiferana爆发严重程度相关.
在Picea abies中,βglu-1转录水平与年度温度范围有关.

结论:

结果表明,与昆虫草食相关的Picea glauca中阿塞托芬防御的潜在适应变异.
影响Picea abies中βglu-1转录变异的因素需要进一步调查,可能涉及气候适应.

关键词:

合唱团的歌手声 (Choristoneura fumiferana) 是一个非常有趣的音乐.皮切亚树 (Picea abies) 是一个树木.皮西亚格劳卡 (Picea glauca) 是一个葡萄树.这是进化反应的进化反应.基因表达的基因表达方式昆虫防护的使用方法

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