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核桃基因组编辑:一个优化的CRISPR/Cas9平台,具有卓越的基因型和内源性促进体

Li Song^1,2, Pu Zhang², Ruixue Gao²

¹State Key Laboratory of Tree Genetics and Breeding, College of Forestry, Northeast Forestry University, Harbin 150040, China.

Horticulture research

|October 23, 2025

概括

研究人员使用优化受体材料和本地促进剂开发了一种高效的核桃 (Juglans regia L.) 基因组编辑平台. 该系统显著提高了CRISPR/Cas9编辑效率,加速了改良的核桃品种的开发.

科学领域:

植物生物技术植物生物技术
基因组学就是基因组学.
农业科学农业科学

背景情况:

核桃 (Juglans regia L.) 在经济上对坚果和木材非常重要.
现有的核桃育种受到生物因素和缺乏高效的基因组编辑系统的阻碍.

研究的目的:

建立一个优化和高效的核桃 (Juglans regia L.) 基因组编辑平台.
为了识别优越的核桃基因型用于基因转换.
为了验证原生核桃促进剂对CRISPR/Cas9基因编辑的有效性.

主要方法:

使用直接的体质胚胎生成和再生,系统地选择优质受体基因型.
使用35S:RUBY在选定的核桃体胚胎中验证基因表达.
克隆和验证了12种坚果特有的内源性Pol III促进体 (JrU3和JrU6).
在CRISPR/Cas9基因编辑中,准了核桃植物脱酶基因 (JrPDS).

主要成果:

核桃品种HT-14被确定为具有高胚胎诱导和再生效率的理想受体材料.
与外源性促进剂相比,核桃特有的内源性促进剂 (JrU3和JrU6) 显著提高了CRISPR/Cas9编辑效率.
JrU3-chr3促进器实现了58.82%的编辑效率,增加了突变频率,同性和多样性.
在HT-14体胚胎中证明了35S:RUBY的成功表达.

结论:

已经建立了一个强大而高效的核桃 (Juglans regia L.) 基因组编辑平台.
原生核桃促进剂为CRISPR/Cas9编辑提供了卓越的性能,加速了功能基因组学和精密育种.
开发的方法为其他多年木质植物的基因组编辑提供了宝贵的见解.

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