在突尼斯的动物牲畜中,分子检测布鲁塞拉物种导致堕胎疫情
Ibtihel Ben Abdallah1, Kaouther Guesmi2, Awatef Béjaoui1
1Group of Bacteriology and Biotechnology Development, Laboratory of Epidemiology and Veterinary Microbiology, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis, Tunisia.
International journal of microbiology
|October 23, 2025
概括
牛病仍然对突尼斯的牲畜构成重大威胁,导致堕胎和经济损失. 这项研究证实了Brucella spp. 使用实时PCR在堕胎的反动物中的存在,识别关键的循环物种和有效的样本类型用于诊断.
科学领域:
- 兽医微生物学 兽医微生物学
- 动物传播疾病 动物传播疾病
- 分子诊断学 分子诊断
背景情况:
- 牛病是突尼斯特有动物传播疾病,在反动物中造成显著的生殖损失,并影响人类健康.
- 尽管采取了控制措施,但牛病仍然存在,因此需要改进诊断策略,以有效管理疾病.
- 由于畜牧业中菌诱导的流产造成的经济损失是巨大的,这凸显了需要准确的检测方法的需要.
研究的目的:
- 评估突尼斯堕胎的绵羊,山羊和牛中布鲁塞拉感染的流行情况.
- 使用差异性qPCR识别循环中的布鲁塞拉物种 (Brucella melitensis和Brucella abortus).
- 确定最适合在堕胎动物中检测布鲁塞拉菌的样品类型.
主要方法:
- 基于IS711的实时PCR测定用于布鲁塞拉spp. 检测. 检测. 在检测.
- 差异性qPCR被用于识别布鲁塞拉美利丁菌和布鲁塞拉堕胎菌.
- 包括阴道扫描,血液,胎盘和胎儿器官在内的样本从2020年到2022年收集和分析.
主要成果:
- 总的来说,24.26%的分析样本检测出Brucella spp.的阳性.
- 在46.96%和19.69%的阳性样本中分别检测到Brucella melitensis和Brucella abortus;在一个案例中观察到共感染.
- 阴道扫描显示了最高的阳性率 (31.13%),这表明它们在诊断牛病中的有用性.
结论:
- 布鲁塞拉种,包括布鲁塞拉美利特尼斯和布鲁塞拉堕胎,正在突尼斯的堕胎的反动物中积极传播.
- 实时PCR和差异性qPCR是准确诊断畜牧动物血清病的宝贵分子工具.
- 有针对性的采样,特别是使用阴道拭子,可以提高诊断灵敏度,并有助于在农场实施有效的控制措施.
相关概念视频
02:04Conservation of Small Populations
Small population sizes put a species at extreme risk of extinction due to a lack of variation, and a consequent decrease in adaptability. This weakens the chances of survival under pressures such as climate change, competition from other species, or new diseases. Large populations are more likely to survive pressures such as these, as such populations are more likely to harbor individuals that have genetic variants that are adaptive under new stresses. Small populations are much less likely to...
01:28Toxoplasmosis
Toxoplasmosis, a zoonotic disease caused by the protozoan Toxoplasma gondii, poses significant public health challenges globally due to its high seroprevalence and varied clinical manifestations. As an obligate intracellular parasite, T. gondii can infect all warm-blooded vertebrates, but felids are its only definitive hosts, shedding unsporulated oocysts into the environment. Humans typically acquire the infection through ingestion of tissue cysts in undercooked meat or oocysts from...