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菌会影响生物膜的结构反应,在剪切应力增加时脱离
Ana Rosa Silva1, C William Keevil2, Ana Pereira1
1LEPABE, ALiCE, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
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概括
剪切应力会影响生物膜脱落和菌在水系统中的释放. 高剪压促使莱吉欧内拉肺进入大量水中的可生存但不可培养状态.
科学领域:
- 环境微生物学环境微生物学
- 水传播病原体研究
- 生物膜的动力学
背景情况:
- 糟糕的水系统管理增加了军团士兵病的风险.
- 生物膜脱落和军团菌的释放与水流变化有关.
- 剪切应力的作用在 legionella 释放和生存能力并未得到充分理解.
研究的目的:
- 为了研究剪切应力对莱吉欧内拉肺生物膜的影响.
- 分析生物膜脱落,病原体释放和可生存但不可培养 (VBNC) 状态转移.
- 了解菌在生物膜结构和稳定性中的作用.
主要方法:
- 在CDC生物膜反应堆中建立了Pseudomonas fluorescens生物膜.
- 带有莱吉欧内拉肺炎和诱导停滞的尖生物膜.
- 在不同剪切应力 (125,225,400 RPM) 恢复流量,并监测生物膜特性和L. pneumophila.
主要成果:
- 带有L. pneumophila的生物膜保持了基底厚度;L. pneumophila粘附在表面上,增强了生物膜的凝聚力.
- 增加的剪切应力促进了生物膜脱落,当军团菌缺席时.
- 所有测试的剪切应力都诱导了L. pneumophila在散装水中的VBNC状态;生物膜中的VBNC细胞发生在400RPM.
结论:
- L. pneumophila的存在会改变生物膜对剪切应力的反应.
- 剪切应力显著影响L. pneumophila的过渡到VBNC状态.
- 结果为水系统中军团菌控制策略提供了洞察力.
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