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单细胞多组特征细胞间通信和细胞内表皮和介质细胞的调节在小鼠的二级口腔发育过程中

Usama Hussein¹, Yulin Dai¹, Andi Liu^1,2

¹Center for Precision Health, McWilliams School of Biomedical Informatics, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.

Computational and structural biotechnology journal

|October 23, 2025

概括

细胞间信号传递,包括WNT,BMP和PDGF通路,对于小鼠的二次口腔发育至关重要. 这些通路在胚胎生长过程中调节细胞通信和组织模式.

科学领域:

发展生物学发展生物学
细胞和分子生物学细胞和分子生物学
基因组学就是基因组学.

背景情况:

器官形态发生依赖于邻近组织之间的细胞-细胞通信.
表皮-介质细胞相互作用通过控制细胞行为来指导二次口腔的发育.
这些相互作用的分子机制尚未完全理解.

研究的目的:

在小鼠的二次口腔发育过程中,研究细胞-细胞通信的分子机制.
在发育中的二级 palatal 细胞中,描述染色质可访问性和基因表达.
确定涉及二级 palatal 形态发生的关键信号通路.

主要方法:

单细胞多基因组测序对35150个来自小鼠发育中的二次口腔细胞进行了测序.
在胚胎日E12.5,E13.5,E14.0和E14.5.5收集样本.
染色体可访问性和基因表达同时进行了分析.

主要成果:

细胞间信号传递对于二次口腔形态发生是关键的.
WNT,BMP和PDGF信号通路是关键的调节器,在E12.5达到峰值,并在E14.5.5下降.
在鼻上皮质中,WNT信号丰富,在口腔上皮质中,BMP丰富,在牙上皮质中,PDGF丰富,协调上皮质-介质细胞相互作用.

结论:

WNT,BMP和PDGF信号通路在二次口腔发育过程中与细胞间通信存在时空联系.
这些途径可能有助于细胞动态,是二级形态发生的基础.
需要进一步的研究,以充分阐明这些机制.

关键词:

蜂通讯蜂通讯是指蜂通讯的形式.基因监管网络基因监管网络帕拉托基尼斯 (Palatogenesis) 是一个古老的发育过程.信号通道的信号通道.单细胞多组体的单细胞多组体.

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