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Updated: Jun 15, 2026

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
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开发一种化学蛋白质平台,以识别复杂蛋白质体内的 (homo) 化位点

Sarah A Mann1, Leonard Barasa2, Paul R Thompson2

  • 1Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, United States.

ACS chemical biology
|October 23, 2025
PubMed
概括

一个新的desthiobiotin-phenylglyoxal (DB-PG) 探针使得特定地点的素化和同素化可以被识别出来. 这种方法有助于我们更好地了解这些蛋白质修饰在诸如风湿性关节炎 (RA) 等疾病中的作用.

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相关概念视频

Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.