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在Cydia pomonella中的宿主特定的嗅觉可塑性:通过嗅觉介导识别核桃挥发物驱动卵巢位置偏好

Peixuan Li¹, Mantao Yang¹, Adil Sattar^1,2

¹College of Forestry and Landscape Architecture, Xinjiang Agricultural University, Urumqi 830052, China.

Journal of agricultural and food chemistry

|October 23, 2025

概括

蝶雌性对核桃和果挥发性物质表现出明显的偏好,格兰酸盐显著影响宿主选择. 这项研究确定了与这些行为有关的关键嗅觉基因,有助于害虫控制策略.

科学领域:

化学生态化学生态学
昆虫学昆虫学是一门学科.
分子生物学分子生物学

背景情况:

植物挥发性物质对于昆虫宿主位置和产卵 (oviposition) 是至关重要的.
鱼 (Cydia pomonella) 是一个重要的农业害虫,影响果和核桃作物.
了解嗅觉反应是管理蝶种群的关键.

研究的目的:

分析和比较来自核桃和果的挥发性化合物.
研究母对这些宿主产卵位置偏好和嗅觉反应的差异.
为了确定宿主特定嗅觉行为的遗传基础.

主要方法:

气色谱-质谱 (GC-MS) 用于从核桃和果中识别挥发性物质.
卵巢位置偏好指数 (OPI) 测试.
电生理学记录 (电子天线图 - EAG) 和单传感录制 (SSR).
定量实时PCR (qPCR) 用于分析嗅觉基因表达.

主要成果:

在核桃中发现了44个挥发性物质,在果中发现了38个.
鱼母表现出不同的卵位偏好和EAG对核桃和果挥发物的反应.
格兰酸在与核桃相关的女性中引起了更强烈的EAG反应和增加了OPI.
在与核桃相关的人群中,特定的嗅觉基因 (cpomSNMP1,cpomOR59,cpomOR35,cpomOR2a,cpomOR5) 被上调.

结论:

格兰酸是核桃相关的蝶的关键吸引剂.
特定的嗅觉基因的升级有助于宿主特定的嗅觉感知.
结果为开发针对性的吸引剂提供了洞察力,用于在各种果园中控制鱼.

关键词:

电天线图 (EAG) 是一种电子天线图.嗅觉的可塑性嗅觉的可塑性排卵行为排卵行为植物挥发性物质是一种挥发性物质.单传感录音 (SSR) 是一种单传感录音.转录组分析转录组分析

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