Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Conserved Binding Sites01:49

Conserved Binding Sites

5.0K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
5.0K
Conserved Binding Sites01:49

Conserved Binding Sites

1.9K
1.9K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

7.1K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.1K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.5K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.5K
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

5.5K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
5.5K
DNA Base Pairing02:27

DNA Base Pairing

32.9K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
32.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Structural Basis of a Novel Heme Binding Bacterial One-Component Switch.

bioRxiv : the preprint server for biology·2026
Same author

Viral transcriptional regulators extensively rewire host pathways through diverse mechanisms.

bioRxiv : the preprint server for biology·2026
Same author

Chromatin accessibility variation provides insights into missing regulation underlying immune-mediated diseases.

eLife·2025
Same author

Beyond the gene: decoding alternative isoforms.

Trends in genetics : TIG·2025
Same author

Detecting transcription factor binding sites with PADIT-seq.

Nature reviews. Genetics·2025
Same author

Missense variants in human forkhead transcription factors reveal determinants of forkhead DNA bispecificity.

Cell reports·2025
Same journal

Tomogram exploration through template matching and deep learning.

Current opinion in structural biology·2026
Same journal

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026
Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026
Same journal

Tuning the physicochemical properties of rationally designed protein-based biomolecular condensates.

Current opinion in structural biology·2026
查看所有相关文章

相关实验视频

Updated: Jan 14, 2026

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

9.2K

TF对应物-DNA结合的自然实验

Shubham Khetan1, Martha L Bulyk2

  • 1Division of Genetics, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.

Current opinion in structural biology
|October 23, 2025
PubMed
概括
此摘要是机器生成的。

转录因子 (TF) 对应物发展出不同的功能,尽管具有相似的DNA结合域. 序列变化和差异性结合部位偏好决定了TF的基因组占用率和调控作用.

更多相关视频

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
08:04

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

9.1K
Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
11:35

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Published on: August 21, 2016

13.4K

相关实验视频

Last Updated: Jan 14, 2026

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

9.2K
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
08:04

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

9.1K
Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
11:35

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Published on: August 21, 2016

13.4K

科学领域:

  • 遗传学 遗传学 是一个
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 转录因子 (TF) 是基因表达的关键调节者.
  • 类似的TF,共享类似的DNA结合域,表现出不同的监管功能.
  • 了解TF功能多样化背后的机制是解读基因调节的关键.

研究的目的:

  • 审查转录因子对应物中的序列变化如何导致功能多样性.
  • 通过遥远的突变来探索DNA结合特异性的全质调制.
  • 讨论竞争性结合动态和低亲缘关系结合位点在TF基因组占用中的作用.

主要方法:

  • 文献综述侧重于转录因子对应物研究.
  • 对序列变异及其对DNA结合特异性的影响的分析.
  • 对竞争性结合动态和TF-DNA相互作用模型的研究.

主要成果:

  • 序列变化,即使是距离DNA接口的距离,也可以在全质上改变TF结合特异性.
  • 类似物联体的共同表达导致复杂的竞争性结合动态.
  • TFs识别了广泛的低亲和度结合位点,有助于形成特定于paralog的结合模式.
  • 对这些地点的差异偏好决定了TF的基因组占用率.

结论:

  • 类似的TF通过微妙的序列差异和各种绑定位置偏好来实现功能多样性.
  • 了解这些机制对于解释遗传变异对基因调节和疾病的影响至关重要.
  • 这种知识有助于预测TF-DNA相互作用及其功能后果.