[对菌体和细菌耐药性的比较研究表明了对物体表面的消毒作用]
1Beijing Center for Disease Prevention and Control, Institute of Disinfection and Pest Control, Beijing 100013, China.
概括
菌体Phi-X174对和酒精消毒剂的耐药性比Staphylococcus aureus和大肠杆菌等细菌更强. 它对四级盐消毒剂的耐药性属于这些细菌.
科学领域:
- 微生物学 微生物学
- 消毒科学 消毒科学
- 病毒学 病毒学
背景情况:
- 消毒有效性通常使用标志性微生物 (如金黄色葡萄球菌和大肠杆菌) 来评估.
- 菌体,如Phi-X174,是感染细菌的病毒,并且越来越多地被考虑用于消毒研究.
- 了解消毒剂耐药性概况对于有效的微生物控制策略至关重要.
研究的目的:
- 为了比较菌 Phi-X174 对常见化学消毒剂的耐药性,与 Staphylococcus aureus 和大肠杆菌的耐药性进行比较.
- 评估含,酒精和四级氨盐消毒剂对这三种微生物剂的疗效.
主要方法:
- 使用定量悬浮试验来评估消毒剂的有效性.
- 测试了三种消毒剂类型:基于 (二化酸盐),基于酒精 (乙醇) 和四级氨盐 (化).
- 在特定的消毒剂度和接触时间下,为菌 Phi-X174 ,金黄色葡萄球菌和大肠杆菌确定了日志减少值.
主要成果:
- 与S. aureus和E. coli相比,Phage Phi-X174表现出明显较低的日志减少值 (表明更高的耐药性),当用二化酸盐和乙醇处理时.
- 对于Phi-X174的日志减少值为3.39 () 和0.03 (乙醇),而对于细菌则为7.25-7.37 () 和2.46-7.30 (乙醇).
- 菲-X174对化的耐药性 (日志减少1.41) 是中等级的,高于大肠杆菌 (0.93),但低于金黄色细菌 (6.84).
结论:
- 菌体Phi-X174在含和基于酒精的消毒剂方面表现出较强的耐药性,与金黄色葡萄球菌和大肠杆菌相比.
- 菌体Phi-X174对四级氨盐消毒剂的耐药性位于S. aureus和E. coli之间的位置.
- 这些发现强调了在选择消毒剂时需要考虑病毒耐药性的必要性,以进行全面的微生物控制.
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