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DNAJC14基因编辑的猪对经典的杀虫病毒有抗性

Helen Crooke¹, Stefanie Schwindt², Sarah L Fletcher³

¹Virology Department, Animal and Plant Health Agency, APHA-Weybridge, Woodham Lane, New Haw, Addlestone, KT15 3NB, UK.

Trends in biotechnology

|October 23, 2025

概括

基因编辑猪来改变DNAJC14宿主蛋白质创造了对古典杀虫病毒的耐药性,如古典猪瘟病毒 (CSFV). 这证明了基因编辑.

科学领域:

兽医病毒学兽医病毒学
分子生物学分子生物学
动物遗传学动物遗传学

背景情况:

传染病严重阻碍了牲畜的生产力和福利.
经典的杀虫病毒,包括经典猪瘟病毒 (CSFV),在农业中造成了巨大的经济损失.
主体蛋白DNAJC14对于古典农虫病毒的复制至关重要.

研究的目的:

为了研究DNAJC14在杀虫病毒复制中的作用.
使用基因编辑技术开发抗疫病毒的牲畜.
评估针对DNAJC14进行疾病控制的有效性.

主要方法:

使用CRISPR/Cas9基因编辑来修改猪的DNAJC14基因.
来自编辑猪的培养初级细胞用于体外病毒复制试验.
被挑战的基因编辑猪携带古典猪瘟病毒 (CSFV) 进行体内疗效评估.

主要成果:

来自DNAJC14表达变化的猪的原始细胞不支持CSFV或牛病毒性腹病毒 (BVDV) 复制.
经过基因编辑的猪对体内CSFV的挑战表现出完全的抵抗力.
证明了DNAJC14在杀虫病毒感染中的重要作用.

结论:

通过CRISPR/Cas9介导的DNAJC14基因编辑,使猪对古典的杀虫病毒产生强大的抵抗力.
通过基因编辑针对宿主-病原体相互作用,为控制牲畜病毒性疾病提供了一个有前途的战略.
这种方法为提高生猪养殖的生物安全性和防止重大经济损失提供了一个新的工具.

关键词:

在 BVDVV 的位置上.克里斯普尔是什么意思?克里斯普尔是什么意思?在CSFVV的CSFV.在DNAJC14中使用.杀虫病毒杀虫病毒杀虫病毒

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