相关实验视频

Updated: Jan 14, 2026

Subnanometer-resolution Structural Determination of Hemagglutinin from Cryo-electron Tomography of Influenza Viruses

Subnanometer-resolution Structural Determination of Hemagglutinin from Cryo-electron Tomography of Influenza Viruses

Published on: November 7, 2025

通过现场冷电子断层扫描可视化A型流感病毒组合

Moritz Wachsmuth-Melm^1,2, Sarah Peterl^1,2, Aidan O'Riain³

¹Department of Infectious Diseases, Virology, Medical Faculty, Heidelberg University, Heidelberg, Germany.

Nature communications

|October 23, 2025

概括

流感A病毒组合涉及膜辅助核糖蛋白复合体 (vRNP) 集群,独立于血凝素 (HA). 矩阵蛋白1 (M1) 在芽之前通过明显的螺旋结构编排组装.

科学领域:

病毒学病毒学
细胞生物学细胞生物学
结构生物学结构生物学

背景情况:

流感A病毒 (IAV) 的组装包括将八个核糖蛋白复合体 (vRNP) 包装成多态粒子.
vRNP 通过 Rab11a 介导的传输到等离子体膜进行分类,以便组装.
矩阵蛋白1 (M1) 在病毒外下调节病毒组合.

研究的目的:

阐明在IAV组装过程中vRNP分布和细胞毒素贩运的分子机制.
了解由M1蛋白组装IAV的协调.
揭示M1蛋白在细胞内组装阶段的结构组织.

主要方法:

在现场冷电子断层扫描 (cryo-ET) 用于可视化IAV组装中间件.
分析的重点是vRNP聚类,膜协会和M1蛋白结构.
研究了依赖Rab11a和独立于HA的VRNP贩运机制.

主要成果:

含有HA的膜作为Rab11a依赖的平台来进行vRNP聚类,减少vRNP之间的距离.
vRNP可以聚集在含有NA的膜上,表明HA独立的组合.
典型的7+1vRNP捆在发芽时形成,由M1层组合协调.
细胞内M1形成独特的多层螺旋组件,与成熟的病毒体中的M1层不同.
这些M1组件在核中是紧的,并在细胞质中部分解离.

结论:

在IAV组装过程中vRNP集群和运输是膜辅助的,但独立于HA.
通过不同的细胞内结构,M1蛋白在协调IAV组装方面发挥着至关重要的作用.
这些发现为流感病毒组装的复杂过程提供了新的分子洞察力.

更多相关视频

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

Published on: July 20, 2022

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

相关概念视频

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...