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[性结肠炎患者的蛋白质生物标志物查和唾液外体的功能分析]

Congyi Yang¹, Xiaowen Zheng², Jingyi Chen¹

¹Department of Gastroenterology, Peking University People's Hospital, Beijing 100044, China.

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences

|October 24, 2025

概括

性结肠炎 (UC) 患者的唾液外体通过激活巨细胞来促进炎症. 这些外体体中的C3,PSMA2,PSMB6和PSMA1等特定蛋白质可以作为UC诊断标记物.

科学领域:

胃肠病学胃肠病学
免疫学免疫学免疫学
蛋白质组学是指蛋白质组学.

背景情况:

性结肠炎 (UC) 是一种慢性炎症性肠病.
唾液外体提供了疾病生物标志物的非侵入性来源.
了解UC病原体需要识别关键的分子参与者.

研究的目的:

确定UC患者与健康对照 (HC) 患者的唾液外体中的差异性蛋白质.
研究UC中高度表达的唾液外体蛋白的功能作用.
探索这些蛋白质作为UC诊断标记物的潜力.

主要方法:

收集了37名活跃UC患者和10名HC患者的全唾液.
使用液体染色体质谱法 (LC-MS/MS) 提取和分析了外体蛋白质.
进行了基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.
使用巨细胞和唾液外体进行了体外共同培养测定,通过ELISA测量炎症性细胞因子分泌 (IL-6,IL-1β,TNF-α) 和通过qPCR测量巨细胞标志物.

主要成果:

与HC相比,在UC唾液外体中确定了11种高度表达的蛋白质和4种低表达的蛋白质.
在UC外体中发现蛋白质酶相关蛋白质 (PSMA1,PSMA2,PSMB6) 和补充因子C3的表达增加.
证明UC唾液外体在体外促进M1巨细胞的两极分化和促炎性细胞因子 (IL-1β,IL-6,TNF-α) 的分泌.

结论:

来自UC患者的唾液外基因组表现出促炎功能.
包括C3,PSMA2,PSMB6和PSMA1在内的特定蛋白质在UC唾液外体中发生显著变化,并与免疫/炎症反应有关.
这些差异表达的蛋白质有潜力作为UC的诊断生物标志物,并可能有助于其病变发生.

关键词:

生物标志物生物标志物肠道免疫肠道免疫是一种免疫系统.唾液外基因组在唾液外基因组中性结肠炎是一种

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