相关实验视频

Updated: Jan 14, 2026

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

一个可光切换的HaloTag用于空间时空控制活细胞中的光

Franziska Walterspiel¹, Begoña Ugarte-Uribe¹, Jonas Weidenhausen¹

¹European Molecular Biology Laboratory, Meyerhofstraße 1, 69117, Heidelberg, Germany.

Angewandte Chemie (International ed. in English)

|October 24, 2025

概括

化学遗传上的可光切换光体为生物成像提供可逆的光控制. 这项新技术使用一种经过修改的HaloTag蛋白来实现精确的,光诱导的跟踪活细胞中的分子.

科学领域:

生物化学生物化学
分子生物学分子生物学
显微镜的使用方法

背景情况:

光敏光对先进的生物成像至关重要,使精确的时空跟踪和超高分辨率显微镜成为可能.
可以反复激活的可逆报道器很少,这限制了活体系统中的动态成像应用.

研究的目的:

开发新的化学遗传可光切换光体,用于生物成像中的可逆光控制.
设计一个可调节的可光开关的HaloTag (psHaloTag) 用于调节使用光的光素排放.

主要方法:

利用HaloTag自我标记蛋白与原性罗达胺染料连接体.
将一个对光敏感的蛋白质域纳入HaloTag,以创建psHaloTag的变体.
在活细胞中测试psHaloTag的光调节性能和与超分辨率显微镜的兼容性.

主要成果:

工程 psHaloTag 变种在活细胞中展示了可逆的,光诱导的光调制 (启动).
在450nm照明下,在各种生物分子点上实现了大而深红的光变化.
与超高分辨率显微镜技术,如单分子局部化显微镜 (SMLM) 证明了兼容性.

结论:

化学遗传方法为设计可调节,可光切换的记者提供了一个多功能平台.
psHaloTag通过基因和合成可修改的可光切换报道器实现了动态成像应用.
这项技术有望在活生物系统中推进分子跟踪和成像.

关键词:

化学遗传学化学遗传学光显微镜的光学显微镜.这是HaloTag的标签.照片交换机照片交换机罗达胺是一种胺.

更多相关视频

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Published on: January 17, 2019

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Published on: April 25, 2018

相关概念视频

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...