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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.6K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.6K
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

5.4K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
5.4K
Membrane Fluidity01:26

Membrane Fluidity

14.5K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
14.5K

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相关实验视频

Updated: Jan 14, 2026

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET
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与功能相关的膜蛋白动力学:整合生物信息学,分子动力学和单分子FRET.

Hugh R Higinbotham1,2, Christine A Arbour2,3, Barbara Imperiali2,3

  • 1Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Protein science : a publication of the Protein Society
|October 24, 2025
PubMed
概括

我们开发了一种结合结构生物信息学,分子模拟和单分子弗斯特共振能量转移 (FRET) 显微镜的新方法来研究膜蛋白动力学. 这种方法揭示了小单一的糖酶转移酶如何在结合联体时改变形状,这对于糖合物生物合成至关重要.

关键词:
糖合物生物合成 糖合物生物合成膜蛋白是一种膜蛋白质.分子动力学分子动力学非规范性氨基酸突变发生的过程.单分子FRETET是一种单分子FRET基质特异性 基质特异性

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Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
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Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

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Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
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Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

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相关实验视频

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Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET
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Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

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Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
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Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

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Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
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科学领域:

  • 结构生物学是结构生物学.
  • 生物物理学的生物物理.
  • 生物化学 生物化学

背景情况:

  • 集成膜蛋白在细胞过程中起着至关重要的作用.
  • 了解它们的结构动态是阐明功能的关键.
  • 小型单一型糖转移酶 (SmPGT) 超级家族对于 prokaryotes 中的糖合物生物合成至关重要.

研究的目的:

  • 开发和应用一套综合策略,以观察位子内不可分割的膜蛋白的依赖于连接体的结构动态.
  • 为了研究SmPGT超级家族内的结构功能关系.
  • 为了验证蛋白质运动在PglC.ligand结合中的作用.

主要方法:

  • 采用结构生物信息学,分子模拟和单分子Förster共振能量转移 (FRET) 显微镜的综合方法.
  • 开发一个平台,用于监测与原生类似的脂质环境中的分子内蛋白质动力学,使用烯-烯酸脂质纳米粒子 (SMALPs).
  • 利用选择性囊蛋白标记,非正规氨基酸突变发生,并点击化学来创建双标记的PglC变体.

主要成果:

  • 在SmPGT超级家族中确定了基质特定的结构特征.
  • 使用全原子模拟的结构特征的相关联联体依赖性构造动力学.
  • 证明在与抑制剂结合时PglC的构造变化与抑制剂强度相关.

结论:

  • 开发的单分子FRET-SMALP策略有效地监测了与原生类似的膜环境中的蛋白质动态.
  • 这种方法适用于研究具有不同基质特异性的多种SmPGT.
  • 结构预测和分子动力学支持在这种蛋白质超级家族中的联体结合时发生显著的构造变化.