Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Childhood Maltreatment and Emotional Intelligence Across Development: A Multilevel Meta-Analytic Review.

Trauma, violence & abuse·2026
Same author

Author Correction: Ultrasensitive Ribo-seq reveals translational landscapes during mammalian oocyte-to-embryo transition and pre-implantation development.

Nature cell biology·2026
Same author

Adverse Childhood Experiences and Subjective Well-Being in Adolescents: The Role of Needs Satisfaction and Self-Compassion.

International journal of psychology : Journal international de psychologie·2026
Same author

The AltR transcription factor responds to plant thiosulfinates to regulate gene expression in a bacterial pathogen of onion.

PLoS pathogens·2026
Same author

Functional urine production and glomerular maturation from embryonic metanephros transplanted at the renal hilum in rats.

Renal failure·2026
Same author

T cell exhaustion: next-generation target of autoimmune diseases?

Journal of leukocyte biology·2026

相关实验视频

Updated: Jan 14, 2026

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
09:43

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Published on: August 10, 2015

16.5K

脱细胞化老鼠管道用于组织工程.

Qianfeng Jia1, Kailin Li2,3, Tongyan Liu4

  • 1Department of Urology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China.

Translational andrology and urology
|October 24, 2025
PubMed
概括

研究人员开发了一种新的方法来创建脱细胞化管支架. 这些支架保留了自然的细胞外基质,为脏组织工程和再生医学提供了一个有前途的平台.

关键词:
脱细胞化 脱细胞化慢性病 (CKD) 是一种慢性病.细胞外矩阵 (ECM) 是一个细胞外矩阵.管 管 管 管组织工程是组织工程.

更多相关视频

Preparation of Decellularized Kidney Scaffolds in Rats
06:09

Preparation of Decellularized Kidney Scaffolds in Rats

Published on: March 18, 2021

4.7K
Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation
08:50

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

Published on: June 9, 2022

2.5K

相关实验视频

Last Updated: Jan 14, 2026

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
09:43

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Published on: August 10, 2015

16.5K
Preparation of Decellularized Kidney Scaffolds in Rats
06:09

Preparation of Decellularized Kidney Scaffolds in Rats

Published on: March 18, 2021

4.7K
Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation
08:50

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

Published on: June 9, 2022

2.5K

科学领域:

  • 生物医学工程 生物医学工程
  • 再生医学是一种再生医学.
  • 组织工程是组织工程.

背景情况:

  • 慢性病 (CKD) 是一个全球性的健康问题.
  • 组织工程提供了使用脱细胞化支架的再生的潜力.
  • 全器官再细胞化带来了重大挑战.

研究的目的:

  • 研究脱细胞化管作为再生的新策略.
  • 为了评估脱细胞化管的再细胞化潜力.
  • 为了克服整个器官再细胞化的局限性.

主要方法:

  • 小切割大鼠管的微切割.
  • 使用0.5%的二硫酸盐 (SDS) 进行脱细胞化.
  • 通过免疫光学,TEM,DNA量化和ELISA对IV原蛋白进行表征.

主要成果:

  • 成功生成了脱细胞化的管支架.
  • 保存的管状底层膜和细胞外矩阵 (ECM) 架构.
  • 使用传输电子显微镜 (TEM) 确认了超结构完整性.

结论:

  • 建立了无细胞管支架的可复制方法.
  • 脚手架保留了原有的ECM特性.
  • 为细胞-ECM相互作用研究,疾病建模和脏组织工程中的药物查提供了一个平台.