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低温增加了通过细胞内 alkalization 的腺病毒复制
Wenwu Sun1, Zhuang Ma1, Jianping Cao1
1Department of Respiratory Medicine, General Hospital of Northern Theatre Command, Shenyang, China.
Frontiers in cellular and infection microbiology
|October 24, 2025
概括
较低的温度通过增加细胞内pH值和促进糖解来增强肺细胞中的腺病毒复制. 抑制糖解或使外部环境酸性化会减少这种效应,揭示温度,细胞代谢和病毒感染之间的联系.
科学领域:
- * 病毒学 病毒学
- * 细胞生物学 * 细胞生物学
- * 环境健康 环境健康
背景情况:
- *呼吸道病毒感染在寒冷的月份更常见.
- *低温对肺上皮细胞和病毒复制的影响尚未完全理解.
- * 腺病毒是呼吸系统疾病的常见原因.
研究的目的:
- * 为了研究低温对A549肺上皮细胞中的腺病毒复制的影响.
- * 确定细胞内pH (pHi) 和葡萄糖溶解在依赖温度的病毒复制中的作用.
主要方法:
- *培养的A549细胞暴露于不同的温度 (33°C和37°C).
- *测量了细胞内pH值 (pHi).
- * 腺病毒复制量得到量化.
- *使用2-脱氧-D-葡萄糖 (2-DG) 或PFK158.8来抑制糖解.
- * 奥利戈米辛用于刺激糖分溶解流.
- * 细胞外介质的pH值得到调整.
主要成果:
- *较低的温度 (33°C) 增加了细胞内pH值 (化) 和腺病毒复制.
- * 抑制糖解减弱了在33°C时增强的病毒复制.
- * 用小菌素刺激糖解,在37°C时增加病毒复制.
- * 低温诱导的病毒复制和化被细胞外酸化抑制.
结论:
- * 低环境温度可以促进腺病毒在肺上皮细胞中的复制.
- *这种增强是由温度诱导的细胞内化和增加的糖分分解介导的.
- * 针对细胞pH和糖解可能提供控制温度依赖的病毒感染的策略.
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