相关实验视频

Updated: Jan 14, 2026

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Published on: October 14, 2022

一个远端调节元件调节Wx基因表达和大米中的粉含量

Qingqing Yang^1,2, Fuhua Dong¹, Wenjie Zhu¹

¹Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou University, Yangzhou, China.

Frontiers in plant science

|October 24, 2025

概括

研究人员确定了一种影响大米中的Waxy (Wx) 基因的远端调节元件 (CRE). 这种CRE显著降低了Wx米中的糖含量和改变了质量,为培育改进的米品种提供了潜力.

科学领域:

植物遗传学和分子生物学
农作物科学农作物科学
食品科学食品科学食品科学

背景情况:

由Waxy (Wx) 基因编码的颗粒结合粉合成酶I (GBSSI) 对于大米中的粉生物合成至关重要.
粉含量显著影响米饭的和食用质量.
虽然已经研究了Wx基因促进体,但远端调节元件仍未得到充分探索.

研究的目的:

识别和表征影响Wx基因表达的远端调节元件 (CREs).
研究一种新的远端CRE对粉含量和米质量特征的影响.
探索这个CRE在米育种方面的潜力.

主要方法:

鉴定和确认与Wx基因相关的远端CRE.
在Nipponbare大米中生成具有Wx^b和Wx^a等位基因的克里基因突变.
对粉糖含量,Wx mRNA和蛋白质水平,凝化温度和粘度的分析.
评价谷物形状,状和主要的农学特征.

主要成果:

一个远端的CRE被确定并证实可以调节Wx基因表达.
与野生类型相比,Wx^b-cre突变体的粉糖含量显著下降 (21.43%-31.51%).
在Wx^a-cre突变体中没有观察到氨含量的显著差异.
Wx^b-cre突变体表现出较低的凝化温度和最终粘度,而不会影响主要的农学特征.

结论:

远端CRE精细调节Wx基因表达和粉素含量,特别是在Wx^b等位基因中.
这种CRE提供了一种有价值的工具,可以通过育种来改善大米的质量特征.
克雷突变可能会诱导粉代谢中的转录重编程.

关键词:

Wxx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx Wx 含有粉糖的含量这是一个 cis-regulatory 元素.谷物质量的谷物质量米米饭米饭米饭米饭.

更多相关视频

Transverse Sectioning of Mature Rice Oryza sativa L. Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

Transverse Sectioning of Mature Rice Oryza sativa L. Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

Published on: January 25, 2022

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

相关概念视频

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...

Regulation of Expression Occurs at Multiple Steps

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

Regulation of Expression Occurs at Multiple Steps

Regulation of Expression Occurs at Multiple Steps

Transcriptional Regulation: Riboswitches

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

Cis-regulatory Sequences

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...