温度对乙型肝炎表面抗原通过牙擦拭剂检测的影响
Hira Tariq1, Mateen Izhar2, Namra Mahmood3
1Pathology/Microbiology, Avicenna Medical College, Lahore, PAK.
Cureus
|October 24, 2025
概括
较高的温度提高了在牙擦片中的乙型肝炎表面抗原 (HBsAg) 检测. 这一发现表明,运输这些诊断样本不需要冷链,简化了乙型肝炎的测试.
科学领域:
- * 病毒学和免疫学
- * 诊断微生物学 * 诊断微生物学
背景情况:
- *乙型肝炎病毒 (HBV) 感染仍然是全球重要的健康问题.
- *准确且易于使用的乙型肝炎表面抗原 (HBsAg) 诊断方法对于有效的管理和预防至关重要.
- * 牙拭片为检测HBsAg提供了采集血液的微创替代方案.
研究的目的:
- * 为了评估高温 (50°C) 与环境温度 (27-32°C) 对HBsAg检测的影响,使用牙拭子.
- * 评估不同温度条件下的牙拭片样本中HBsAg的稳定性.
- * 确定是否需要冷链来运输HBsAg阳性牙拭片.
主要方法:
- * 一项非随机对照研究,涉及138名HBsAg阳性和138名HBsAg阴性个体.
- * 收集每位参与者两份牙拭片,不包括口腔健康问题的人.
- * 24小时内在27-32°C和50°C的酸盐缓冲盐水中化拭子,然后进行HBsAg检测的自动ELISA.
主要成果:
- *HBsAg在50°C化的138个阳性抽样中的108个被检测到,而在27-32°C化的84个抽样中检测到.
- *所有138个已知的阴性抹布在两种温度下均呈阴性,证实了特异性.
- *在50°C的升高温度下观察到更高的检测率.
结论:
- *高温 (50°C) 显著提高了从牙拭片中检测HBsAg的检测率.
- * 用于HBsAg测试的牙拭片样本在更高的温度下是稳定的,因此在运输过程中不需要冷链.
- * 这种方法为在资源有限的环境中进行HBsAg查提供了一个有希望的,简化的方法.
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