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呼吸速率作为基于X射线的生物标志物,用于纵向评估肺功能和病理
Kaveh Ahookhosh1,2, Willy Gsell1, Birger Tielemans1,3
1Biomedical MRI, Department of Imaging and Pathology, KU Leuven, Leuven, Belgium.
Frontiers in medicine
|October 24, 2025
概括
我们开发了一种使用微CTX射线数据的非侵入性方法,用于监测小鼠的呼吸速率 (RR). 这种技术准确地测量肺功能和病理,有助于临床前研究,并提高了人类研究的可翻译性.
科学领域:
- 生物医学工程 生物医学工程
- 肺部医学 肺部医学
- 医疗成像医学成像
背景情况:
- 呼吸速率 (RR) 是临床前研究中未充分利用的肺功能参数.
- 微计算机断层扫描 (microCT) 提供空间和时间肺部数据,使生物标志物提取成为可能.
- 非侵入性,纵向监测RR可以提高动物和人类研究之间的可翻译性.
研究的目的:
- 开发一种非侵入性管道,用于使用微CT X射线投影进行纵向呼吸率 (RR) 监测.
- 在临床前模型中确定RR作为肺功能和病理学的生物标志物.
- 为了验证肺上皮损伤的小鼠模型中的管道.
主要方法:
- 使用机械通风小鼠 (60-185bpm) 的微型CT扫描作为基本事实.
- 从X射线投影中提取原始强度曲线,包含呼吸和心脏信号.
- 应用信号处理技术,包括Lomb-Scargle (LS) 算法,以隔离和分析呼吸信号.
主要成果:
- 伦布-斯卡格尔算法证明了强大的RR预测,错误率为3%.
- 开发的管道成功检测了小鼠损伤模型中的过渡性肺功能损失,通过减少RR表示RR.
- 在受伤恢复期间,观察到肺总体和空气肺体积的同时增加.
结论:
- 基于X射线的管道允许非侵入性,同时收集形态和功能性肺部数据.
- 纵向RR监测是肺部疾病进展和修复的宝贵生物标志物.
- 这种方法有助于将临床前发现转化为临床试验.
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