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表达,净化和ATP水解活动测量CtISWI和CtATPase的表达,净化和ATP

Yiming Zhao1,2, Jingjun Hong1,3,2

  • 1Institute of Health Sciences and Technology (IHST), Institutes of Physical Sciences and Information Technology, Anhui University, Hefei, China.

Current protocols
|October 24, 2025
PubMed
概括

这项研究详细介绍了一种快速协议,用于表达和净化大肠杆菌中的仿制SWITch (ISWI) 蛋白质. 该方法产生了大量的可溶性蛋白质,用于研究染色质重塑和ATP水解活性.

关键词:
通过ATP的水解.亚太酶 ATPase 是一种酶.染色体重塑器 染色体重塑器这就是FPLC.在ISWIWI中使用.

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相关概念视频

Caspases01:24

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside cells.