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Updated: Jan 6, 2026

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使用SpyCatcher-SpyTag固定的Leloir-glycosyltransferases进行糖甘合成

Philip Palm1, Kai Philip Hussnaetter1, Lothar Elling2

  • 1Laboratory for Biomaterials, Institute for Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany.

Applied microbiology and biotechnology
|October 24, 2025
PubMed
概括

本研究介绍了一种使用SpyCatcher-SpyTag技术的新型酶固定方法,用于高效合成人乳寡糖化物 (HMO). 这种技术提高了酶的稳定性和可重复使用性,使复杂的HMO结构的成本效益高的生产成为可能.

关键词:
酶性糖甘合成的方法固定化 固定化 固定化在 Leloir 葡萄糖基转移酶.马莱胺激活的阿加罗斯这就是SpyCatcher.这就是SpyTag.

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