通过整合WGCNA和机器学习来识别急性冠状动脉综合征的新型生物标志物
Jie Zheng1, Fan Gong2, Liping Zhu3
1Department of Cardiology, Tianyou Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, China.
IET systems biology
|October 24, 2025
概括
这项研究确定了关键的免疫细胞变化和分子生物标志物ARG1,HECW2和PFKFB3,与急性冠状动脉综合征 (ACS) 进展有关. 这些发现为ACS提供了潜在的新疗法和诊断工具.
科学领域:
- 心血管科学 心血管科学
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
背景情况:
- 早期动脉样硬化病变中的免疫细胞有助于炎症和急性冠状动脉综合征 (ACS).
- 炎症和ACS进展之间的确切关系尚不清楚.
- 了解这些分子机制对于开发有效的ACS治疗至关重要.
研究的目的:
- 在ACS.中识别差异表达的信使RNA (mRNA) 和microRNA (miRNA).
- 发现潜在的生物标志物用于ACS诊断和预后.
- 阐明免疫细胞透在ACS病变发生过程中的作用.
主要方法:
- 从基因表达综合 (GEO) 数据库中分析mRNA和miRNA表达特征.
- 生物信息分析包括基因组丰富分析 (GSEA),权重基因同表达网络分析 (WGCNA),LASSO和支持矢量机-递归特征消除 (SVM-RFE).
- 针对免疫细胞透的CIBERSORT分析和针对miRNA-mRNA相互作用网络构建的Cytoscape.
主要成果:
- 确定了98个差异表达的mRNA和627个miRNA.
- 发现了ARG1,HECW2和PFKFB3作为潜在的ACS生物标志物.
- 揭示了免疫细胞种群的变化,CD4记忆休息T细胞减少,单细胞和中性粒细胞增加.
- 在ARG1,HECW2,PFKFB3和特定的免疫细胞类型之间展示了密切的相关性.
结论:
- ARG1,HECW2和PFKFB3被确定为ACS的潜在诊断生物标志物.
- 免疫细胞失调,特别是涉及单细胞和中性粒细胞,在ACS中发挥着重要作用.
- 这些发现为ACS病原和潜在的治疗点提供了新的见解.
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