Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

17.9K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
17.9K
Viral Mutations00:36

Viral Mutations

39.6K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
39.6K
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

18.6K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
18.6K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

6.2K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
6.2K
Viral Recombination00:57

Viral Recombination

24.9K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
24.9K
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

14.5K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
14.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

IQ-TREE 3: phylogenomic inference software using complex evolutionary models.

Molecular biology and evolution·2026
Same author

Panmap: Scalable phylogeny-guided alignment, genotyping, and placement on pangenomes.

bioRxiv : the preprint server for biology·2026
Same author

WEPP: Phylogenetic placement achieves near-haplotype resolution in wastewater-based epidemiology.

PLoS computational biology·2026
Same author

Rate variation and recurrent sequence errors in pandemic-scale phylogenetics.

Nature methods·2026
Same author

Addressing pandemic-wide systematic errors in the SARS-CoV-2 phylogeny.

Nature methods·2026
Same author

The 340B Drug Pricing Program, Hospital Prices, and Competition in Commercial Markets.

Health services research·2026
Same journal

Population Epigenetics: Deciphering DNA Methylation Diversity and its Implications for Health, Disease, and Evolution.

Molecular biology and evolution·2026
Same journal

Genomic signature of repeated transitions to diurnality in spiders.

Molecular biology and evolution·2026
Same journal

Phylogenomic blind spots: The limits of UCE and BUSCO loci in the presence of gene flow.

Molecular biology and evolution·2026
Same journal

seqLens: Optimizing Language Models for Genomic Predictions.

Molecular biology and evolution·2026
Same journal

The transcriptional and translational outcomes for pseudogenes in bacterial endosymbionts.

Molecular biology and evolution·2026
Same journal

800 million years of co-evolution in the green plant lineage - the case of LEUNIG and SEUSS transcriptional co-regulators.

Molecular biology and evolution·2026
查看所有相关文章

相关实验视频

Updated: Jan 14, 2026

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
05:22

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion

Published on: September 13, 2024

1.2K

在SARS-CoV-2中高度重复的多核酸突变.

Nicola De Maio1, Olivier Anoufa1,2, Kyle Smith3

  • 1European Molecular Biology Laboratory-European Bioinformatics Institute, EMBL-EBI, Wellcome Genome Campus, Hinxton, Cambridgeshire CB10 1SD, UK.

Molecular biology and evolution
|October 24, 2025
PubMed
概括
此摘要是机器生成的。

在SARS-CoV-2中某些多核酸突变是惊人的常见,而不是随机的. 大多数与转录调节有关,这表明病毒复制过程中存在模板切换机制.

关键词:
这就是SARS-CoV-2病毒.复杂的突变 复杂的突变多核酸突变的多核酸突变流行病规模的家族遗传学经常发生的突变是反复发生的突变.

更多相关视频

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.5K
Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
18:10

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Published on: June 16, 2011

30.0K

相关实验视频

Last Updated: Jan 14, 2026

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
05:22

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion

Published on: September 13, 2024

1.2K
Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.5K
Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
18:10

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Published on: June 16, 2011

30.0K

科学领域:

  • 基因组学就是基因组学.
  • 病毒学 病毒学
  • 进化生物学 进化生物学

背景情况:

  • 多核酸突变,同时改变多个DNA基,在进化和疾病中至关重要.
  • 它们通常被认为是罕见和随机的遗传事件.
  • 了解它们的模式对于精确的基因组分析和疾病研究至关重要.

研究的目的:

  • 研究SARS-CoV-2基因组中多核酸突变的频率和模式.
  • 为了确定驱动反复复的多核酸突变的潜在机制.
  • 评估这些突变对基因组数据分析的影响,特别是在进化研究中.

主要方法:

  • 分析了超过200万个公开可用的SARS-CoV-2基因组.
  • 识别和表征复发的多核酸突变模式.
  • 突变部位和已知的基因组特征之间的相关性分析,例如转录调节序列.
  • 对计算进化生物学工具的影响评估,特别是重组推断.

主要成果:

  • 在SARS-CoV-2中发现了高度复发的多核酸突变,在各个血统中独立发生了数百次.
  • 发现97.6%的这些反复发生的突变与转录调节序列有关.
  • 建议在病毒转录期间采用模板切换机制作为可能的原因.
  • 证明反复复发的多核酸突变导致大约12%的SARS-CoV-2重组推断中的错误阳性.

结论:

  • 经常发生的多核酸突变是SARS-CoV-2进化的重要,非随机特征.
  • 与转录相关的模板切换为这些观察到的突变模式提供了合理的解释.
  • 这些发现需要对分析病毒演变的计算方法进行调整,并可以提高基因组数据解释的准确性.