在小鼠血中评估rubusoside的药理动力学特征和生物可用性,使用UPLC-MS/MS分析
Meiling Zhang1, Siyu Zhuo2, Fangmei Zhang2
1School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
概括
这项研究使用了超高性能液体色谱-并联质谱法 (UPLC-MS/MS) 来分析小鼠中的rubusoside药理动力学. 该方法被证明是有效的评估rubusoside.
科学领域:
- 药理学和药理动力学 药理学和药理动力学
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 鲁布索化是一种具有潜在治疗应用的化合物.
- 了解其药理动力学特征对于药物开发至关重要.
- 需要准确的量化方法来研究rubusoside的行为 in vivo.
研究的目的:
- 开发和验证一种超高性能液体染色学-并联质谱法 (UPLC-MS/MS) 方法,用于量化小鼠血中的鲁布索化物.
- 在小鼠口服和静脉注射后评估rubusoside的药理学特性.
主要方法:
- 血样本是使用蛋白质沉与酸制备的.
- 使用UPLC HSS T3柱与渐变化实现了色谱分离.
- 使用UPLC-MS/MS检测和定量鲁布索化物,以异coparin作为内部标准.
主要成果:
- 该UPLC-MS/MS方法表现出极好的线性 (3.51120 ng/mL),精度 (<7%) 和准确性 (92.3108.1%).
- 康复率超过74.2%,矩阵效应在可接受的范围内 (107.1110.0%).
- 在测试剂量下,rubusoside的绝对口服生物利用率约为1%.
结论:
- 经过验证的UPLC-MS/MS方法适用于rubusoside的药理动力学研究.
- 鲁布索西德在小鼠中表现出较低的口服生物利用性.
- 进一步的研究可以利用这种方法来探索rubusoside的治疗潜力.
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