鉴定食用产生的新化合物
Katarzyna Bierla1, Marek Siwulski2, Laurent Ouerdane1
1Universite de Pau, E2S, CNRS, Institute of Analytical and Physical Chemistry for the Environment and Materials (UMR 5254), Hélioparc, 64053 Pau, France.
Food chemistry
|October 24, 2025
概括
像Pleurotus ostreatus这样的食用能有效地代谢,特别是 (Se(IV),将其转化为有机形式. 这项研究揭示了中的新代谢物,增强了我们对生物可用性的理解.
科学领域:
- 菌群学 菌群学是指菌群学的学科.
- 生物化学 生物化学
- 环境科学 环境科学
背景情况:
- 是一种必不可少的微量元素,其生物可用性取决于其化学形式.
- 众所周知,食用会积累和转化,但代谢途径尚未完全阐明.
- 了解真菌中的代谢对于评估食摄入量和生物可用性至关重要.
研究的目的:
- 为了研究Pleurotus ostreatus和Hericium coralloides的代谢能力.
- 为了比较从化石 (Se(IV)) 和酸 (Se(VI)) 吸收的效率.
- 为了识别和描述这些真菌物种中形成的有机物种.
主要方法:
- 用和酸盐种植.
- 测量吸收效率的测量方法.
- 阳离子交换高性能液体染色学与诱导合等离子体质谱学 (HPLC-ICP-MS) 结合用于特种化.
- 水友交互液体染色学 (HILIC) 与电子喷射电离质谱学 (ESI-MS) 用于表征水溶性代谢物.
主要成果:
- 与酸 (Se(VI)) 相比,酸的吸收率是酸 (Se(IV)) 的4-8倍.
- 观察到Se(IV) 完全 (>98.5%) 代谢为有机形式,而8-18%的Se(VI) 仍然未被代谢.
- 在水溶性物种 (>40%),多糖类 (~20%),蛋白质 (~10%) 中发现了代谢.
- 甲是P. ostreatus中占主导地位的物种,而H. coralloides的丰度较低.
- 希利克-ESI-MS确定了10种额外的水溶性代谢物,包括单氨酸衍生物和含乙烯基-单氨基的二.
结论:
- 斑点鱼和Hericium coralloides有效地将化物代谢成各种有机化合物.
- 这项研究确定了新的代谢物,表明中以前未知的代谢途径.
- 这些发现有助于理解在真菌中的生物转化及其对在人类饮食中的生物可用性的影响.
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