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血样本准备方法的最新发展,用于用液态染色学质谱仪进行有针对性的代谢学研究

Phaedra Verding¹, Yvan Vander Heyden², Ann Van Eeckhaut³

¹Vrije Universiteit Brussel (VUB), Faculty of Medicine and Pharmacy, Research group Analytical Chemistry, Applied Chemometrics and Molecular Modelling (FABI), Laarbeeklaan 103, Brussels 1090, Belgium; Vrije Universiteit Brussel (VUB), Faculty of Medicine and Pharmacy, Research group Experimental Pharmacology (EFAR), Center for Neurosciences (C4N), Laarbeeklaan 103, Brussels 1090, Belgium.

Journal of pharmaceutical and biomedical analysis

|October 24, 2025

概括

本综述探讨了针对目标代谢学的先进样本制备技术,重点是针对有限的血样本采用具有高提取效率的小型化方法. 新的策略克服了传统方法的局限性,改善了临床前研究中的代谢物分析.

科学领域:

分析化学分析化学
生物化学生物化学
生物技术是生物技术.

背景情况:

目标代谢学量化特定的代谢物以了解生物状态.
通过液态染色学和质谱学进行等离子体分析,需要做好样本准备.
临床前研究面临的挑战是有限的样本量和低代谢物度.

研究的目的:

审查和批判性地评估针对目标代谢学微型样本制备方法.
突出传统方法的局限性,并引入新的战略.
评估灵感来自非目标代谢学的替代方法的潜力.

主要方法:

对针对性代谢组的样本准备技术的文献综述.
讨论传统方法 (如液体色谱-质谱) 和它们的缺点.
探索包括SPE,MEPS,3D打印 Sorbents,MIPs,磁纳米粒子和各种微提取技术在内的新策略.

主要成果:

传统方法的样本清理不良,选择性低,提取时间长,使用危险的溶剂等问题.
新的技术,如固相微提取,磁性纳米颗粒,和微提取通过包装的剂提供了提高效率和小型化.
这些先进的方法解决了有限的样本量和低度的分析物所带来的挑战.

结论:

具有高提取效率的小型样本制备对于有限的血样本的向代谢学至关重要.
新型策略显著改善了传统方法,使得更好的代谢物量化.
进一步探索替代技术可能会增强血代谢学分析.

关键词:

液体染色学质谱学微提取微提取微提取在等离子体中,我们可以得到等离子体.样品的准备样品的准备有针对性的代谢组学.

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