酸盐限制对PHBV生产和微生物群落动态的影响,这些微生物群落被用合成发酵消化剂养
Alessio Castagnoli1, Sitihaya Malibo2, Tianshi Li3
1Department of Energy, Systems Territory and Construction Engineering, University of Pisa, Via C.F. Gabba 22, Pisa, 56122, Italy.
Journal of environmental management
|October 24, 2025
概括
在测序批量反应堆 (SBR) 中,优化碳与 (C/P) 比率是聚酸 (PHA) 生产的关键. 适度的C/P比增强了PHA的积累和微生物群体的稳定性.
科学领域:
- 环境微生物学环境微生物学
- 生物技术是生物技术.
- 生物反应器工程 生物反应器工程
背景情况:
- 微生物的多基酸盐 (PHA) 生产受营养的可用性影响.
- 了解碳与 (C/P) 比率对微生物群落的影响对于优化生物反应器性能至关重要.
研究的目的:
- 调查不同C/P比如何影响微生物群体动态和PHA生产在测序批量反应器 (SBR).
- 为了确定最大限度地增加PHA积累和保持反应堆稳定性的最佳C/P比.
主要方法:
- 在三种不同的C/P比率 (170,235和400 Cmol Pmol−1) 下在SBR中培养微生物群落.
- 对PHA积累,生物质增长和反应堆稳定性的分析.
- 使用全长16S/18S rRNA基因测序 (PacBio) 的高分辨率微生物社区概况.
- 显微镜分析 (TEM) 来确认PHA颗粒和多酸盐储量.
主要成果:
- 适度的C/P比 (235 Cmol Pmol−1) 导致了最高的PHA体积生产率 (2.02 g PHA L−1 d−1) 和工艺稳定性.
- 较高的C/P比率增加了PHA储存产量,但降低了生产率,并损害了污泥沉降能力和反应堆稳定性.
- 微生物群落分析显示了细菌和真核生物多样性的转变,中等的C/P比率有利于多样化的PHA积累细菌和稳定原体群落.
- 在低于最佳条件下观察到Sphaerotilus的主导地位,这可能与反应堆膨胀有关.
结论:
- C/P比率极大地影响混合微生物培养的性能和PHA的产生.
- 优化C/P比对于提高PHA产量和生产率至关重要,同时确保微生物群落的稳定性.
- 细胞的多样性,特别是细菌食和掠食性原生体,可能通过选择性掠食来维持系统稳定性.
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