相关实验视频

Updated: Jan 14, 2026

Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites

Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites

Published on: March 16, 2018

一个分泌的Leishmania金属蛋白酶通过准DICER1-miRNA通路来操纵宿主铁的调节

Suman Samanta¹, Sourav Banerjee¹, Rupak Datta¹

¹Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, Mohanpur, West Bengal, India.

The Journal of biological chemistry

|October 24, 2025

概括

大型寄生虫Leishmania使用分泌的GP63来降解宿主Nramp1蛋白质. 这种机制涉及向DICER1和miR-122,增加寄生虫的铁可用性.

科学领域:

免疫学免疫学免疫学
寄生虫学的寄生虫学
分子生物学分子生物学

背景情况:

宿主细胞使用Nramp1 (自然抗性相关的巨细胞蛋白1) 来封存铁,限制细胞内病原体的生长.
大型莱什马尼亚通过通过肝素介导途径降解Nramp1来规避这种防御.
人们怀疑寄生虫分泌的因素会影响宿主铁代谢.

研究的目的:

为了确定负责Nramp1降解的Leishmania主要分泌因子.
阐明这种因素影响宿主铁平衡的分子机制.
调查这种机制在莱什曼尼亚感染中的作用.

主要方法:

用莱什马尼亚条件介质处理的巨细胞培养物.
对Nramp1蛋白水平和肝素表达的分析.
寄生虫金属蛋白酶GP63.3的鉴定和表征
对DICER1和miR-122水平的评估.
在体内使用Leishmania GP63淘汰杆菌在BALB/c小鼠中的研究.

主要成果:

莱什马尼亚主要条件介质诱导了巨细胞中的Nramp1降解和肝素表达.
寄生虫金属蛋白酶GP63被确定为这种效应的关键媒介.
发现GP63消耗了巨细胞DICER1,影响了miR-122的成熟.
在体外和体内,GP63的淘汰菌株未能诱导肝素或降解Nramp1.
感染了GP63淘汰赛菌株的小鼠显示了减少的Nramp1抑制.

结论:

大型Leishmania利用分泌的GP63来操纵宿主铁的可用性.
GP63的目标是宿主DICER1/miR-122通路,以诱导肝素和降解Nramp1.
这种寄生虫策略增强了铁的获取,促进了Leishmania的生存.

关键词:

DICER1 的意思是说.在 GP63 GP63 里面.第1章没有了在SLC11A1A1中.铁是铁,铁是铁,铁是铁.莱什曼尼亚莱什曼尼亚据报道,MIR-1222号机器人是一架飞机.

更多相关视频

In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice

In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice

Published on: February 20, 2020

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Published on: September 13, 2024

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...