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破坏膜的中链脂肪酸减少金黄色葡萄球菌在抗生素治疗期间持续细胞存活率
Yena Seo1, Minjun Kim1, Dae-Youn Kim1
1Department of Forest Products and Biotechnology, College of Science and Technology, Kookmin University, Seoul, Republic of Korea.
中链脂肪酸 (MCFA) 有效地杀死金黄色葡萄球菌的持续性细胞,这些细胞在慢性感染中难以治疗. 劳里克酸通过破坏细菌膜表现出最强烈的活性.
科学领域:
- 微生物学 微生物学
- 皮肤病学 皮肤病学
- 传染性疾病 传染性疾病
背景情况:
- 持久性细胞是细菌的休眠亚种群,可以在抗生素治疗中存活,从而导致慢性感染和抗生素耐药性.
- 中链脂肪酸 (MCFA) 通常存在于局部产品中,并具有抗菌性质.
- 黄金葡萄球菌 (Staphylococcus aureus) 是一个重要的人类病原体,经常导致持久性感染.
研究的目的:
- 评估MCFA在消灭金黄色葡萄球菌持续性细胞方面的有效性.
- 研究MCFA消除持久细胞的作用机制.
主要方法:
- 评估了各种MCFA对S. aureus持久性菌的杀菌活性,先用抗生素 (西普洛克萨,牛素,托布拉米) 进行治疗.
- 使用光显微镜和ATP泄漏试验分析了膜透性.
- 研究了膜流动性和跨膜潜力的变化.
主要成果:
- 八酸,十酸和酸显著降低了抗生素存活的黄金色细菌在低毫米度下持续存在.
- MCFA的杀菌活性随着脂肪酸链的长度 (从酸到酸) 的增加而增加.
- 杀死活性与膜透性增加相关,但与膜流动性或潜力的变化无关.
结论:
- 多重重脂肪酸,特别是酸,是有效的消灭黄金色细菌的有效药物,可能有助于治疗慢性感染.
- 该机制涉及破坏细菌膜的透性.
- 与代谢活跃细胞不同,米里斯酸对持久性细胞无效,表明不同的生理状态.
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