Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Improving Translational Accuracy02:07

Improving Translational Accuracy

14.1K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
14.1K
Real Time RT-PCR02:57

Real Time RT-PCR

64.6K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
64.6K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

7.1K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
7.1K
Complementary DNA01:44

Complementary DNA

31.2K
Overview
31.2K
Transfer RNA Synthesis02:35

Transfer RNA Synthesis

3.5K
3.5K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

13.2K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
13.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Understanding Size Distributions during Lipid Nanoparticle Manufacturing through Mechanistic Modeling.

ACS omega·2026
Same author

Modular Design of Vacuum Systems for Lyophilization.

Industrial & engineering chemistry research·2026
Same author

Systems analysis of the kinetics of in vitro transcription from interactions of T7 RNA polymerase and DNA.

Archives of biochemistry and biophysics·2026
Same author

Continuous lyophilization of suspended vials with per-vial inline analytics.

Journal of pharmaceutical sciences·2026
Same author

Model-Based Optimization of Fed-Batch In Vitro Transcription.

Chembiochem : a European journal of chemical biology·2025
Same author

Manufacturing mRNA-Loaded Lipid Nanoparticles with Precise Size and Morphology Control.

ACS nano·2025

相关实验视频

Updated: Jan 14, 2026

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

10.7K

端到端的数字双胞胎软件用于连续的mRNA制造.

Mohammed Aatif Shahab1, Nathan Merica Stover1, Hasan Al-Mahayni1

  • 1Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139, USA.

International journal of pharmaceutics
|October 24, 2025
PubMed
概括

一个新的数字双胞胎软件通过模拟关键的生产步骤,使信使RNA (mRNA) 疗法的连续制造成为可能. 这加快了流程优化,并支持可扩展,可靠,数据驱动的生产,以满足全球卫生需求.

关键词:
连续的生物制造.数字双胞胎 数字双胞胎 数字双胞胎开源软件是开源的软件.过程模拟的过程模拟.它们是mRNARNA.

更多相关视频

Optimization of In vitro Transcription Reaction for mRNA Production Using Chromatographic At-Line Monitoring
07:04

Optimization of In vitro Transcription Reaction for mRNA Production Using Chromatographic At-Line Monitoring

Published on: April 4, 2025

1.9K
Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

12.9K

相关实验视频

Last Updated: Jan 14, 2026

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

10.7K
Optimization of In vitro Transcription Reaction for mRNA Production Using Chromatographic At-Line Monitoring
07:04

Optimization of In vitro Transcription Reaction for mRNA Production Using Chromatographic At-Line Monitoring

Published on: April 4, 2025

1.9K
Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

12.9K

科学领域:

  • 生物技术是生物技术.
  • 制药制造业 制药制造业 制药制造业
  • 计算机建模 计算建模

背景情况:

  • 使者RNA (mRNA) 疗法提供了快速开发和广泛的应用,但面临着与传统批量工艺的制造可扩展性和一致性问题.
  • 连续制造为提高mRNA生产效率和可靠性提供了一个有希望的替代方案.

研究的目的:

  • 开发和展示一种新的数字双胞胎软件,用于端到端连续制造mRNA疗法.
  • 将关键单元操作集成到一个模块化,用户友好的平台,用于过程模拟和优化.

主要方法:

  • 该软件集成了体外转录 (IVT),触流过 (TFF),连续染色学 (CCTC),脂质纳米粒子 (LNP) 配方和冷干燥模块.
  • 图形界面允许工程师在没有编码的情况下操纵模块,由机械模型和数据存储数据库支持.
  • 基于莫里斯的全球灵敏度分析用于流程优化和模型验证.

主要成果:

  • 该软件成功地模拟了所有五个单元的操作,在一个代表性的mRNA生产场景中进行了模拟.
  • 生成的时间序列和3D图为流程动态和产品特性提供了洞察力.
  • 灵敏度分析确定了流程优化关键输入因素.

结论:

  • 数字双胞胎软件加速了过程优化,并促进了mRNA制造的质量设计 (QbD) 原则.
  • 它为闭环控制和操作员培训提供了一个平台,支持数据驱动的连续制造.
  • 这项技术提高了mRNA疗法生产的可扩展性,可靠性和响应性.