含有黄素的奇托/聚乙烯胺胺纳米凝促进抗菌和抗氧化活性
Khatereh Asadi1, Kourosh Seddigh Ardakani2, Dordaneh Ghaffaripour3
1Guilan Road Trauma Research Center, Trauma Institute, Guilan University of Medical Sciences, Rasht, Iran; Medical Biotechnology Research Center, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran.
International journal of biological macromolecules
|October 24, 2025
概括
新的奇托/聚乙烯胺纳米凝封装黄素 (CUR-Ngs),以提高其稳定性和传递性. 这些CUR-Ngs表现出增强的细胞活力,减少氧化应激,以及对MRSA和VRE等耐药病原体具有强大的抗菌活性.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术 纳米技术
- 药理学 药理学是指药理学的学科.
背景情况:
- 黄素 (CUR) 对慢性疾病具有有益的特性,但其稳定性和生物可用性不佳.
- 自由CUR的局限性包括需要高剂量和潜在的长期暴露副作用.
- 开发有效的药物输送系统对于增强CUR的治疗潜力至关重要.
研究的目的:
- 为了合成和描述用于封装黄素 (CUR) 的新奇奇桑/聚乙烯胺纳米凝 (Ngs).
- 评估CUR-装载纳米凝 (CUR-Ngs) 的稳定性,受控释放,生物相容性和治疗疗效.
- 评估CUR-Ngs作为抗菌剂对抗关键抗生素耐药性病原体的潜力.
主要方法:
- CUR被封装在生物相容的奇托/聚乙烯胺纳米凝中.
- 描述包括富里埃变换红外光谱 (FTIR) 和电子显微镜用于形态和尺寸分析.
- 确定了负载能力 (LC) 和封装效率 (EE).
- 进行了体外释放研究,细胞活力测试 (HUVEC,HGF),氧化应激标志物 (LDH,ROS,LPO) 和抗菌活性 (MIC).
主要成果:
- 成功合成了平均直径为115.71nm的球形CUR-Ngs,显示出良好的光谱兼容性.
- CUR-Ngs的装载能力为9.4%,封装效率为68.1%.
- 在96小时内观察到持续释放的特征,增强了细胞活力和显著减轻了HUVEC细胞中的氧化应激.
- 在CUR-Ngs检测中,针对黄金葡萄球菌 (Staphylococcus aureus),肠杆菌 (Enterococcus faecalis),MRSA和VRE.有强烈的最低抑制度 (MIC) 值.
结论:
- 奇托/聚乙烯胺纳米凝为增强CUR稳定性,生物可用性和受控释放提供了有效的平台.
- CUR-Ngs表现出极好的生物相容性,减少了氧化应激,并对抗多药耐药细菌具有显著的抗菌疗效.
- 这些发现表明,CUR-Ngs在治疗慢性疾病和对抗抗生素耐药病原体引起的与医疗相关的感染方面具有相当大的前景.
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