单细胞RNA测序和空间转录组的整合揭示了急性损伤中中性粒细胞的多样性和空间异质性

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Xintong Liu¹, Zehua Li¹, Lichao Mao¹

¹Renal Division, Peking University Institute of Nephrology, Peking University First Hospital, Beijing, China; Key Laboratory of Renal Disease-Ministry of Health of China, Key Laboratory of CKD Prevention and Treatment (Peking University)-Ministry of Education of China, Peking University First Hospital, Beijing, China; Beijing Key Laboratory of Precision Medicine and New-drug/Equipment Development for Severe Kidney Disease, China; Research Units of Diagnosis and Treatment of Immune-mediated Kidney Diseases-Chinese Academy of Medical Sciences, Peking University First Hospital, Beijing, China.

International journal of biological macromolecules

|October 24, 2025

概括

这项研究揭示了中性粒细胞在急性损伤 (AKI) 中的不同作用,确定了新的亚型和独特的中性粒细胞-相互作用. 这些发现为诊断和治疗AKI提供了新的途径.

科学领域:

腎臟病學 (nephrology) 是一種醫學專業.
免疫学免疫学免疫学
基因组学就是基因组学.

背景情况:

急性损伤 (AKI) 是一种严重的疾病,治疗方法很少.
中性粒细胞是AKI的关键参与者,但它们的多样性和作用尚未得到充分理解.

研究的目的:

在AKI中使用先进的转录基因技术全面绘制中性粒细胞异质性的地图.
确定新型中性粒细胞亚种群及其在AKI病变发生中的功能作用.
探索AKI微环境中的中性粒细胞的空间相互作用及其预后影响.

主要方法:

在各种AKI模型中使用了整合性单细胞和空间转录组学.
确定了九种不同的中性粒细胞亚种群,包括新型.
进行了空间邻近分析和与人类AKI预后的相关性.

主要成果:

在AKI中新描述了六个中性粒细胞亚种群,其中三个是新鲜的.
中性粒细胞表现出功能异质性,参与细胞化,抗原呈现,炎症和纤维化.
发现了一种新的中性粒细胞为中心的微环境,涉及与厚的上升四肢 (TAL) 相互作用.
中性粒细胞子集和微环境与人类AKI的长期预后不佳相关.

结论:

这项研究提供了AKI中中性粒细胞多样性的详细地图.
新型中性粒细胞子集和相互作用提供潜在的预后生物标志物.
这些发现支持开发针对中性粒细胞的治疗方法来治疗AKI.

关键词:

急性损伤是什么?急性损伤是什么?免疫微环境是一个微环境.中性粒细胞中性粒细胞.空间转录组学空间转录组学我们的 scRNAseqq.

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