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通过两个MALDI-MS系统对牛奶细菌进行比较分析

Jagoda Pałczyńska^1,2, Oleksandra Pryshchepa³, Agnieszka Ludwiczak^1,4

¹Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Toruń, Poland.

Scientific reports

|October 24, 2025

概括

两种矩阵辅助飞行质谱仪 (MALDI-TOF MS) 的激光脱吸/电离时间系统,Bruker和Zybio,在常规微生物诊断中显示出可比的有效性,用于识别生牛奶中的细菌菌株.

科学领域:

微生物学微生物学
分析化学分析化学
质谱测量质量谱测量

背景情况:

准确的细菌鉴定对于食品安全和诊断至关重要.
飞行质谱的矩阵辅助激光脱吸/离子化时间 (MALDI-TOF MS) 是一种快速有效的细菌识别技术.
对不同MALDI-TOFMS系统的比较研究对于优化诊断工作流程至关重要.

研究的目的:

为了比较两个MALDI-TOF MS系统的识别准确度:布鲁克微LT生物型和Zybio EXS2600 Ex-Accuspec.
评估从生牛奶中分离出来的细菌分离物的两种系统在属和物种水平上的性能.
评估两种系统在细菌识别方面的一致性.

主要方法:

从生牛奶中分离出来的细菌菌株的比较分析.
使用两个 MALDI-TOF MS 系统进行识别:布鲁克微 LT 生物型和Zybio EXS2600 Ex-Accuspec.
采用管内提取方法进行样本制备.
总共鉴定了 1,130 个细菌分离物.

主要成果:

布鲁克系统实现了94.6%的基因层识别;Zybio系统实现了91.3%的基因层识别.
布鲁克和齐比奥系统在物种层面上约有75%的一致性.
在大约25%的案例中,在属和物种层面观察到差异.

结论:

布鲁克和齐比奥MALDI-TOFMS系统在生牛奶中的细菌识别方面都表现出高性能.
这些系统在常规微生物诊断中表现出相似的有效性.
可能需要进一步调查,以解决大约25%的识别中的差异.

关键词:

马尔迪-托夫 (MALDI-TOF) 是一个国家元首.质谱测量质量谱测量微生物微生物牛奶中的细菌.

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