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低温-EM结构分析揭示了CDC48展开酶的植物特异性适应

Brandon Huntington¹, Anandsukeerthi Sandholu¹, Jun Wang¹

¹KAUST Center of Excellence for Smart Health, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.

Plant communications

|October 25, 2025

概括

植物CDC48A蛋白质降解与动物和真菌不同,利用更具适应性的辅因子系统. 这项研究揭示了Arabidopsis thaliana CDC48A独特的结构和功能特征,这表明有针对性蛋白质降解的祖先机制.

科学领域:

分子生物学分子生物学
生物化学生物化学
进化生物学进化生物学

背景情况:

向蛋白质降解对于蛋白质组的稳态和真核生物的适应至关重要.
在这个过程中,CDC48展开酶复合体起着中心作用.
虽然动物和真菌CDC48系统的特征很好,但植物的适应性在很大程度上仍未被探索.

研究的目的:

为了阐明Arabidopsis thalianaCDC48A.的结构和功能特征.
研究AtCDC48A与其辅因子UFD1和NPL4.4的相互作用.
了解植物CDC48介导的蛋白质降解的进化背景.

主要方法:

在不同状态下对Arabidopsis thalianaCDC48A的结构性确定.
对AtCDC48A与UFD1和NPL4辅因子复合的分析.
功能性测试以评估辅因子相互作用和目标降解.
对CDC48系统的比较进化分析.

主要成果:

在CDC48A,与其动物和酵母同类相比,它显示出不同的域动态.
AtNPL4与AtCDC48A具有独特的相互作用,并且可以独立调解目标降解.
AtNPL4和AtUFD1不形成强制性异构体,允许协同作用.
这些植物特异性特征在植物物种中得到保护,可能代表祖先的真核生物状态.

结论:

阿拉比多普西斯塔利亚纳 (Arabidopsis thaliana) CDC48A在辅因子参与中表现出独特的结构和功能适应.
工厂CDC48系统采用了一个更模块化和组合的辅助因子使用策略.
这突出了植物向蛋白质降解的专门机制,可能反映了祖先的真核生物模式.

关键词:

在AAA+ ATPase中.适应适应适应适应低温电磁波冷却器 (Cryo-EM) 是一个非常好的方法.植物蛋白质质量控制有针对性的蛋白质降解降解.无处不在的蛋白酶体系统

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