基于等离子光热的手术工具和病原体悬浮物的快速杀菌
Sarabjot Kaur1, Parminder Kaur2, Shobit Attery3
1CSIR-Central Scientific Instruments Organisation, Sector 30-C, Chandigarh 160030, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Journal of photochemistry and photobiology. B, Biology
|October 25, 2025
概括
这项研究表明,等离子体光热灭菌有效地消除了使用银纳米板和可见近红外光在手术工具上的病原体. 这种快速的低温方法可以实现可重复使用的仪器的高绝育保证水平.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术纳米技术
- 传染病控制和控制传染病
背景情况:
- 手术工具的灭菌对于预防与医疗相关的感染至关重要.
- 现有的方法可能耗时,需要高温或苛刻的化学物质.
- 像ESKAPE这样的广谱病原体在临床环境中构成重大威胁.
研究的目的:
- 研究用于快速杀菌手术工具和病原体悬浮物的等离子体光热技术的有效性.
- 用合成的银纳米板来评估ESKAPE病原体的高负载的消除.
- 展示可重复使用的外科器械的新,高效和安全的灭菌方法.
主要方法:
- 合成三角形银纳米板并用可见近红外 (可见-NIR) 光照射它们.
- 将等离子体光热技术应用于受污染的手术工具和病原体悬浮液 (Candida albicans,每种5 × 10^7 CFU/mL).
- 开发了一种内部辐射源和灭菌装置原型,用于在BALB/C小鼠体内测试.
主要成果:
- 在10分钟内在低温 (∼65-70°C) 实现快速杀菌.
- 证明了高绝育效率,每种病原体的Log10减少>7.69,超过6Log10绝育保证水平 (SAL).
- 证实了纳米板的无菌性和多个循环的可重复使用性;观察到形态变化,膜溶解和ROS生成.
结论:
- 等离子光热技术为手术工具和病原体悬浮提供了高度有效和快速的灭菌方法.
- 该技术是安全的,具有成本效益和环保,具有广泛临床应用的潜力.
- 这项研究是首次报告使用等离子体光热技术对可重复使用的外科工具进行灭菌的案例.
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