唾液采集方法和储存条件影响唾液度:最佳唾液采样和储存的建议
Fei Li1, Duan Li1, Hexi Zhang1
1Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University, Tianjin, China.
The Journal of nutrition
|October 25, 2025
概括
建议在冲洗5分钟后通过自然分泌物收集唾液,以准确测量唾液度 (SIC). 对于长期储存 (≥180天),将样品保存在-80°C;短期储存 (≤90天) 允许-80°C,-20°C或4°C.
科学领域:
- 生物化学 生物化学
- 营养科学 营养科学
- 分析化学 分析化学
背景情况:
- 唾液度 (SIC) 是评估营养的关键指标.
- 缺乏收集和储存唾液的标准化协议,影响SIC测量可靠性.
研究的目的:
- 评估不同唾液收集和储存方法对SIC的影响.
- 为可靠的SIC评估建立标准化的协议.
主要方法:
- 健康的成年人在各种刺激和口水条件下提供唾液样本.
- 样品在-80°C,-20°C和4°C下保存长达365天.
- 在不同的时间点测量SIC水平以评估稳定性.
主要成果:
- 刺激和立即冲洗后显著改变了SIC.
- 唾液样本在 -80°C保存时保持稳定365天.
- 在-20°C和4°C的储存导致了随着时间的推移显著的SIC降解,特别是在180天后.
结论:
- 冲洗5分钟后自然分泌是推的收集方法.
- 长期储存 (≥180天) 需要在-80°C保存.
- 短期储存 (≤90天) 在-80°C,-20°C或4°C是可行的.
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