解读微生物多样性和预测发酵色素水中的代谢功能,使用培养独立的表征
Shruti Mishra1, Asif Ali Vadakkethil2, Mir Asif Iquebal2
1Department of Interdisciplinary Sciences, National Institute of Food Technology Entrepreneurship and Management, Kundli, Sonipat 131028, Haryana, India.
Journal of microbiological methods
|October 25, 2025
概括
这项研究探讨了从有色米制成的调味发酵水 (FFRW) 的微生物和植物化学特征. 它揭示了储存期间微生物新陈代谢和多含量的变化,突出了FFRW.
科学领域:
- 食品科学和微生物学
- 营养生物化学 营养生物化学
- 发酵技术的发酵技术
背景情况:
- 发酵水 (FRW) 已被公认为具有传统意义和潜在的健康益处.
- 来自有色素米品种 (黑色,棕色,红色) 的调味发酵米水 (FFRW) 具有丰富的营养和功能性质.
- FFRW的微生物组成和代谢动态在很大程度上仍未被描述.
研究的目的:
- 用分子测序技术研究FFRW中的细菌和真菌多样性.
- 分析FFRW冷藏储存期间的植物化学成分和代谢变化.
- 阐明FFRW作为功能饮料的功能和代谢潜力.
主要方法:
- 使用16S rRNA基因测序进行细菌多样性分析.
- 使用内部转录间隔器 (ITS) 测序进行真菌多样性分析.
- 通过非目标分析和代谢途径预测 (KEGG) 进行植物化学分析.
主要成果:
- 在黑色FFRW中观察到显著的细菌多样性差异,在储存期间在棕色和红色FFRW中观察到稳定性.
- 主导的微生物群落包括乳酸细菌 (韦塞拉菌) 和各种酵母.
- 代谢途径表明,在30天内,从氨基酸降解转向碳水化合物和脂肪酸代谢.
- 棕色FFRW呈现出更高的聚醇含量,在储存30天后观察到减少.
结论:
- FFRW拥有多样化的细菌和真菌群落,具有显著的代谢潜力.
- 微生物的新陈代谢和植物化学成分在储存期间发生变化,影响FFRW的功能属性.
- 由于其丰富的微生物和植物化学特征,FFRW显示出作为功能饮料的潜力.
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