循环自然杀手T (NKT) 类细胞的年龄匹配参考值

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Updated: Jun 19, 2026

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Published on: October 30, 2016

Elena Trombetta¹, Manuela Liguori¹, Federico Simone Colombo¹

¹Flow Cytometry Laboratory, Clinical Pathology, Fondazione IRCCS ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.

Scandinavian journal of immunology

|October 27, 2025

概括

天然杀手T型 (NKT型) 细胞,对于先天免疫力至关重要,表现出与年龄相关的变化. 这项研究确定了成人和儿童NKT类细胞群的参考值,有助于诊断免疫相关疾病.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
临床诊断临床诊断临床诊断

背景情况:

自然杀手T型 (NKT型) 细胞是关键的细胞毒性淋巴细胞,参与天生的免疫力.
类似NKT细胞分布的不平衡与各种病理有关,包括癌症和自身免疫性疾病.
确定NKT类细胞群的年龄匹配参考值对于准确的临床解释至关重要.

研究的目的:

确定成人和儿童NKT样细胞数和亚种群的年龄匹配参考值.
研究NKT类细胞分布的年龄相关趋势,包括CD4和CD8表达.
为在临床诊断中利用NKT样细胞数据提供基础.

主要方法:

使用溶解无洗协议进行流细胞计,分析了2208名受试者的NKT类细胞.
根据6个年龄段的490名健康成人和儿科对照组的数据,确定了基准值.
评估NKT类细胞数量和CD4/CD8表面抗原的表达.

主要成果:

随着年龄的增长,NKT样细胞数量 (百分比和绝对数量) 会增加.
随着年龄的增长,NKT样/CD8+细胞增加,而NKT样/CD4+细胞减少.
双阳性 (CD4+CD8+) NKT 样细胞随着年龄的增长而减少,而双阴性 (CD4-CD8-) 细胞在成年人减少之前增加到16岁.

结论:

这项研究在CE-IVD条件下为NKT类细胞亚群提供了第一个可靠的,年龄匹配的基准值.
定义的参考范围对于识别与各种疾病相关的NKT类细胞计数异常至关重要.
将NKT样细胞数据纳入诊断报告可以提高患者免疫概况的划分.

关键词:

类似NKT的细胞.淋巴细胞淋巴细胞参考值是指指标值的参考值.

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