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通过组合性转录基因分析和WGCNA,探索激情果皮中安东基氨酸的合成

Hui-Ying Chen^1,2, Fan-Li Gao¹, Quan Guo¹

¹Guangxi Key Laboratory of Electrochemical and Magneto-chemical Functional MaterialsCollege of Chemistry and Bioengineering, Guilin University of Technology, Guilin, China.

Frontiers in plant science

|October 27, 2025

概括

激情果皮含有有价值的抗生素. 这项研究揭示了调节激情果 (Passiflora edulis) 皮肤中安东素积累的关键基因,为利用这种废弃生物质提供了洞察力.

科学领域:

植物分子生物学植物分子生物学
农业科学农业科学
生物技术是生物技术.

背景情况:

激情果 (Passiflora edulis) 纸被广泛使用,但皮皮通常被丢弃作为废物.
激情果皮含有丰富的抗素,有价值的生物活性化合物.
不充分利用激情果皮意味着有价值的生物质的损失.

研究的目的:

调查激情果皮中安东素积累的分子机制.
鉴定不同品种和发育阶段的激情果生物合成中涉及的关键基因.
探索激情果皮的潜力,作为安东素的来源.

主要方法:

综合性转录组和权重基因联合表达网络分析 (WGCNA).
在成熟和不成熟阶段的紫色和黄色激情果品种的转录组测序.
使用DESeq2进行差异基因表达分析,并通过KEGG,GO和KOG数据库进行基因注释.
使用RT-qPCR验证候选枢纽基因.

主要成果:

鉴定出与氨酸生物合成相关的差异表达基因 (DEGs).
WGCNA确切地确定了十个候选枢纽基因,这些基因可能会调节氨酸积累.
关键基因的验证表达模式,包括PeCA,PeMYC2,PeMYB88和PeCHI,与转录组数据有很强的一致性.

结论:

这项研究阐明了激情果皮中安东素积累的分子基础.
确定了关键的调节基因 (PeCA,PeMYC2,PeMYB88,PeCHI),这些基因对安托素生物合成至关重要.
提供了改善安东素生产和提升激情果皮废物的基础.

关键词:

RT-qPCRR 是一个人.在WGCNA中,WGCNA是WGCNA.它们是安托西安氨酸.激情的果实是一种激情的果实.转录组分析转录组分析

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