从基因组到现场:Bacillus halotolerans BJ-3作为葡萄藤Botryosphaeria衰退的新型生物控制溶液
Manawaduge Ayesha Madushani1,2,3, Yonghua Li1, Xinghong Li1
1Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Key Laboratory of Environment Friendly Management on Fruit and Vegetable Pests in North China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, China.
Current research in microbial sciences
|October 27, 2025
概括
一种新型细菌菌株,Bacillus halotolerans BJ-3,显示出作为抗葡萄树干疾病的生物杀剂的巨大潜力. 这项研究证实了其在控制Lasiodiplodia theobromae方面的有效性,为葡萄种植提供了可持续的解决方案.
科学领域:
- 植物病理学 植物病理学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 葡萄树干疾病 (GTD),特别是由Lasiodiplodia theobromae引起的Botryosphaeria死亡,严重影响全球葡萄园.
- 现有的GTD控制方法往往是有限的,需要开发可持续的替代方案.
研究的目的:
- 开发一种用于GTD的生物控制剂,使用局部分离的细菌菌株BJ-3.
- 评估BJ-3对L. theobromae和其他GTD病原体的基因组生物控制潜力和有效性.
主要方法:
- 遗传学分析确定了BJ-3菌株为Bacillus halotolerans. 这种菌株具有高分量耐受性.
- 基因组挖掘揭示了11个抗微生物生物合成基因集群.
- 在体外测试中评估了对19种真菌病原体的对抗性.
- 子抑制,葡萄树接种和实地实验验验证了对L. theobromae的有效性.
主要成果:
- 细菌哈洛托伦BJ-3表现出对GTD病原体的广泛抗性,抑制率高达91.62%.
- 在预防性射击治疗中,BJ-3实现了98.47%的L. theobromae子抑制和74.39%的控制.
- 实地试验证实了BJ-3的有效性,实现了71.94%的玻尿菌死亡控制.
结论:
- 细菌哈洛托伦BJ-3是一种有前途的生物真菌杀菌剂,用于控制葡萄藤中博菌的衰退.
- 这项研究为开发B. halotolerans BJ-3衍生的抗菌代谢物为未来的生物控制策略提供了基础.
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