相关实验视频

Updated: Apr 14, 2026

A Protocol for Using Förster Resonance Energy Transfer (FRET)-force Biosensors to Measure Mechanical Forces across the Nuclear LINC Complex

A Protocol for Using Förster Resonance Energy Transfer (FRET)-force Biosensors to Measure Mechanical Forces across the Nuclear LINC Complex

Published on: April 11, 2017

福斯特共振能量转移指标Pyronic在神经元中的双点校准协议

Felipe Baeza-Lehnert¹, Yasna Contreras-Baeza², Camila Aburto²

¹Leipzig University, Carl-Ludwig-Institute of Physiology, Faculty of Medicine, Leipzig, Germany.

|October 27, 2025

概括

研究人员开发了一种新协议,用于测量神经元中的细胞溶性pyruvate度. 该方法使用Pyronic光指示器进行细胞代谢的定量分析.

科学领域:

细胞的新陈代谢
神经科学是一个神经科学.
生物化学生物化学

背景情况:

酸盐是一种关键的代谢中间体,将糖解与呼吸联系起来.
精确测量酸盐对于了解细胞能量生产至关重要.
现有的方法缺乏单细胞分辨率或定量准确性.

研究的目的:

介绍一个详细的协议,以量化细胞质酸盐在神经元中的度.
使用基于Förster共振能量转移 (FRET) 的指标实现单细胞分辨率.
为了建立一个最少的侵入性,两点校准方法.

主要方法:

使用了基因编码的光指示器Pyronic.
采用一种非侵入性的药理两点校准方法.
通过基质操纵和抑制确定Pyronic的动态范围 (ΔRMAX).
纳入Pyronic的体外Kd值.

主要成果:

能够在神经元中测量绝对细胞溶性pyruvate度.
提供了一种定量参数,用于在不同条件下比较pyruvate代谢.
在神经元中证明了Pyronic可靠的校准.

结论:

开发的协议提供了一种可靠和定量方法来研究酸盐代谢.
这种方法适用于神经元中的各种生理和病理情景.
方便对高精度的酸盐代谢进行比较分析.

关键词:

在Pyronic中使用Pyronic.校准校准的时间光是一种光.代谢代谢代谢代谢神经元神经元的神经元皮鲁酸是一种酸盐.量化量化量化量化量化量化量化量化量化量化量化量化量

更多相关视频

Förster Resonance Energy Transfer Measurements in Living Plant Cells

Förster Resonance Energy Transfer Measurements in Living Plant Cells

Published on: June 28, 2021

Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features

Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features

Published on: March 16, 2022

相关概念视频

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...