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Updated: Jan 14, 2026

Conversion of Human Induced Pluripotent Stem Cells iPSCs into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors
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Conversion of Human Induced Pluripotent Stem Cells iPSCs into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors

Published on: May 1, 2019

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从人类诱导的多能干干细胞迅速和简化诱导脊髓运动神经元

YaoYao Chen1, Fan Yang2, Timothy O'Brien3

  • 1Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, China.

Bio-protocol
|October 27, 2025
PubMed
概括

这项研究提出了一种有效的方法,可以在18天内产生人类诱导的多能干细胞衍生的运动神经元 (hiPSC-MN). 这种优化的协议加速了运动神经元疾病研究和治疗开发.

关键词:
不同化的差异化单层培养的文化是一种单层培养.多电极阵列是一个多电极阵列.脊柱运动神经元的运动神经元这是 hiPSC.

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
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