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Updated: Jan 14, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
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在Drosophila中 lncRNA基因表达的Ribozyme-Mediated Knockdown 在Drosophila中

Kevin G Nyberg1, Richard W Carthew1,2

  • 1Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA.

Bio-protocol
|October 27, 2025
PubMed
概括

研究人员开发了一种新型的自我切割的 ribozyme 方法,用于有效的长非编码RNA (lncRNA) 在 Drosophila. 这种基于CRISPR的方法实现了超过90%的核和细胞质 lncRNAs 的敲击效率,超过了RNA干扰 (RNAi) 的限制.

关键词:
这就是CRISPR/Cas9的作用.这种植物是Drosophila.长非编码RNA是什么意思通过RNA的淘汰,RNA的淘汰.利博酶是一种 ribozyme.在 lncRNA 中,

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